Biomarkers for assessing chronic toxicity of carbamazepine, an anticonvulsants drug on Pangasianodon hypophthalmus (Sauvage, 1878)

•The rate of behavioural disorder increases with exposure time and CBZ concentration.•CBZ exposure showed significant reduction in TEC, PCV, Hb level and an increase in TLC.•SGOT, SOD, CAT, cholesterol are the most potential organ-based biomarkers for assessing CBZ toxicity.•Genotoxicity study revea...

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Bibliographic Details
Published in:Environmental toxicology and pharmacology 2021-10, Vol.87, p.103691, Article 103691
Main Authors: Droma, Dawa, Kumar, Saurav, Paul, Tapas, Pal, Prasenjit, Saharan, Neelam, Kumar, Kundan, Poojary, Nalini
Format: Article
Language:English
Subjects:
Carbamazepine
Integrated biomarker response
Micronucleus
Oxidative stress
P. hypophthalmus
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Summary:•The rate of behavioural disorder increases with exposure time and CBZ concentration.•CBZ exposure showed significant reduction in TEC, PCV, Hb level and an increase in TLC.•SGOT, SOD, CAT, cholesterol are the most potential organ-based biomarkers for assessing CBZ toxicity.•Genotoxicity study reveals low concentration of CBZ can potentiate formation of micronucleus in fish. In recent times, carbamazepine (CBZ) as an anticonvulsants drug has raised attention because of its safety concern in the aquatic environment. The present study aimed to evaluate the sub-lethal effects of CBZ (1%, 0.1 % and 0.01 % of 96 h LC50) on P. hypophthalmus for 60 days based on haematological, biochemical, and genotoxicity biomarkers. Chronic exposure of CBZ altered blood profiles (total erythrocyte count, packed cell volume, haemoglobin) and serum biomarkers such as alkaline phosphates, cholesterol, lactate dehydrogenase and transaminase enzymes. Oxidative stress biomarkers such as superoxide dismutase (SOD) and catalase (CAT) activity were also substantially affected in all treatments. Genotoxicity study revealed the formation of micronucleus in erythrocytes of exposed fish. Integrated Biomarker Response (IBR) study showed cholesterol, serum glutamic oxaloacetic transaminase (SGOT) in serum and SOD, CAT in liver tissue are the best organ-based enzyme biomarkers. The present report concludes that an environmentally realistic concentration of CBZ can pose a serious threat to aquatic organisms.
ISSN:1382-6689
1872-7077
DOI:10.1016/j.etap.2021.103691