Soybean phenolic-rich extracts inhibit key-enzymes linked to type 2 diabetes (α-amylase and α-glucosidase) and hypertension (angiotensin I converting enzyme) in vitro

• Published in: Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2013-03, Vol.65 (3), p.305-309
• Main Authors: Ademiluyi, Adedayo O., Oboh, Ganiyu
• Format: Article
• Language: English
• Subjects: alpha-Amylases - antagonists & inhibitors; Angiotensin I converting enzyme (ACE); Angiotensin-Converting Enzyme Inhibitors - isolation & purification; Angiotensin-Converting Enzyme Inhibitors - pharmacology; Antioxidants - isolation & purification; Antioxidants - pharmacology; Diabetes Mellitus, Type 2 - enzymology; Dose-Response Relationship, Drug; Enzyme; Enzyme Inhibitors - isolation & purification; Enzyme Inhibitors - pharmacology; Glycine max - chemistry; Glycoside Hydrolase Inhibitors; Hypertension; Hypertension - enzymology; Peptidyl-Dipeptidase A - metabolism; Phenolics; Phenols - isolation & purification; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Soybean; Type 2 diabetes; α-Amylase; α-Glucosidase
• ISSN: 0940-2993; 1618-1433
• DOI: 10.1016/j.etp.2011.09.005

This study sought to assess the inhibitory activities of phenolic-rich extracts from soybean on α-amylase, α-glucosidase and angiotensin I converting enzyme (ACE) activities in vitro. The free phenolic extract of the soybean was obtained by extraction with 80% acetone, while that of the bound phenolic extract was done by extracting the alkaline and acid hydrolyzed residue with ethyl acetate. The inhibitory action of these extracts on the enzymes activity as well as their antioxidant properties was assessed. Both phenolic-rich extracts inhibited α-amylase, α-glucosidase and ACE enzyme activities in a dose dependent pattern. However, the bound phenolic extract exhibited significantly (P