p,p′-DDE, a DDT metabolite, induces proinflammatory molecules in human peripheral blood mononuclear cells “in vitro”

In a recent study, our group demonstrated that when peripheral blood mononuclear cells (PBMCs) were treated “in vitro” with p,p′-DDE, a DDT metabolite, an antioxidant response and biomarkers of inflammation were induced at the mRNA level, indicating a proinflammatory state. Thus, the aim of this stu...

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Published in:Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2013-07, Vol.65 (5), p.661-665
Main Authors: Cárdenas-González, Mariana, Gaspar-Ramírez, Octavio, Pérez-Vázquez, Francisco J., Alegría-Torres, Jorge Alejandro, González-Amaro, Roberto, Pérez-Maldonado, Iván N.
Format: Article
Language:English
Subjects:
Adult
antioxidants
biomarkers
Blotting, Western
Cell Culture Techniques
Cell Survival - drug effects
Cell Survival - immunology
Cells, Cultured
COX-2
Cytokines - biosynthesis
Cytokines - immunology
DDE
DDT
DDT (pesticide)
DDT - metabolism
Dichlorodiphenyl Dichloroethylene - toxicity
Dose-Response Relationship, Drug
Environmental Pollutants - toxicity
gene expression
Humans
IL-1β
Inflammation
interleukin-1beta
interleukin-6
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - immunology
messenger RNA
metabolites
mononuclear leukocytes
risk
TNF-α
tumor necrosis factor-alpha
Young Adult
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Summary:In a recent study, our group demonstrated that when peripheral blood mononuclear cells (PBMCs) were treated “in vitro” with p,p′-DDE, a DDT metabolite, an antioxidant response and biomarkers of inflammation were induced at the mRNA level, indicating a proinflammatory state. Thus, the aim of this study was to evaluate the induction of proinflammatory molecules at the protein level in PBMCs exposed to p,p′-DDE “in vitro”. The main finding was that “in vitro” exposure to p,p′-DDE enhanced the expression of proinflammatory cytokines (TNF-α, IL-1β, IL-6) at the protein level in PBMCs. We also observed COX-2 induction at the protein level. Considering that p,p′-DDE has been identified as a persistent metabolite and is frequently found in the population, it is important to evaluate early inflammation biomarkers in populations exposed to DDT and to estimate the true risk of inflammatory disease development.
ISSN:0940-2993
1618-1433
DOI:10.1016/j.etp.2012.07.006