Amelioration of titanium dioxide nanoparticles-induced liver injury in mice: Possible role of some antioxidants

This study investigates the efficacy of idebenone, carnosine and vitamin E in ameliorating some of the biochemical indices induced in the liver of titanium dioxide nanoparticles (TiO2 NPs) intoxicated mice. Nano-anatase TiO2 (21nm) was administered (150mg/kg/day) for 2 weeks followed by the aforemen...

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Bibliographic Details
Published in:Experimental and toxicologic pathology : official journal of the Gesellschaft für Toxikologische Pathologie 2015-04, Vol.67 (4), p.305-314
Main Authors: Azim, Samy A. Abdel, Darwish, Hebatallah A., Rizk, Maha Z., Ali, Sanaa A., Kadry, Mai O.
Format: Article
Language:English
Subjects:
Animals
Antioxidants
Antioxidants - pharmacology
Apoptosis
Chemical and Drug Induced Liver Injury - prevention & control
Immunohistochemistry
Inflammation
Liver
Liver - drug effects
Male
Metal Nanoparticles - toxicity
Mice
Oxidative stress
Reverse Transcriptase Polymerase Chain Reaction
Titanium - toxicity
Titanium dioxide nanoparticles
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Summary:This study investigates the efficacy of idebenone, carnosine and vitamin E in ameliorating some of the biochemical indices induced in the liver of titanium dioxide nanoparticles (TiO2 NPs) intoxicated mice. Nano-anatase TiO2 (21nm) was administered (150mg/kg/day) for 2 weeks followed by the aforementioned antioxidants either alone or in combination for 1 month. TiO2 NPs significantly increased serum liver function enzyme activities, liver coefficient and malondialdehyde levels in hepatic tissue. They also suppressed hepatic glutathione level and triggered an inflammatory response via the activation of macrophages and the enhancement of tumor necrosis factor-α and interleukin-6 levels. Moreover, the mRNA expression of nuclear factor-erythroid-2-related factor 2, nuclear factor kappa B and Bax was up-regulated whereas that of Bcl-2 was down-regulated following TiO2 NPs. Additionally, these NPs effectively activated caspase-3 and caused liver DNA damage. Oral administration of idebenone (200mg/kg), carnosine (200mg/kg) and vitamin E (100mg/kg) alleviated the hazards of TiO2 NPs with the combination regimen showing a relatively higher effect. The histopathological examination reinforced these findings. In conclusion, oxidative stress could be regarded as a key player in TiO2 NPs-induced liver injury. The study also highlights the anti-inflammatory and the anti-apoptotic potentials of these antioxidants against the detrimental effects of TiO2 NPs.
ISSN:0940-2993
1618-1433
DOI:10.1016/j.etp.2015.02.001