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Increased plasma EPO and MIP-1α are associated with recruitment of vascular progenitors but not CD34(+) cells in autologous peripheral blood stem cell grafts

Objective Increased levels of endothelial-like vascular progenitor cells (VPCs) in peripheral blood stem cell (PBSC) products have been associated with reduced transplant-related toxicity following autologous hematopoietic stem cell transplantation. In this study, a panel of angiogenic and inflammat...

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Published in:Experimental hematology 2009-06, Vol.37 (6), p.673-678
Main Authors: Labonté, Laura, Li, Yuhua, Yang, Lin, Gillingham, Akira, Halpenny, Michael, Giulivi, Antonio, Sills, Terrence, Evans, Kenneth, Zanke, Brent, Allan, David S
Format: Article
Language:English
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Summary:Objective Increased levels of endothelial-like vascular progenitor cells (VPCs) in peripheral blood stem cell (PBSC) products have been associated with reduced transplant-related toxicity following autologous hematopoietic stem cell transplantation. In this study, a panel of angiogenic and inflammatory plasma proteins were quantitatively analyzed in patients undergoing PBSC collection for autologous hematopoietic stem cell transplantation to identify profiles associated with greater VPC recruitment. Materials and Methods A panel of 16 candidate plasma factors were quantified using multianalyte fluorescence and/or enzyme-linked immunosorbent assay. VPC clusters were enumerated using a standard cell culture assay. Results Thirty-six patients (mean age = 51 years, 42% female) had plasma collected at baseline prior to PBSC mobilization and on the day of PBSC collection. Only erythropoietin (EPO) levels increased significantly on the day of PBSC collection in comparison with baseline plasma levels (2.2-fold increase; p = 0.003). Interleukin-2, -10, epidermal growth factor, interferon-α, and angiopoietin-1 all decreased significantly between baseline and the day of PBSC collection ( p < 0.02). The remaining cytokine levels did not change appreciably ( p = NS). The cohort was divided into “low” graft VPCs (
ISSN:0301-472X
1873-2399
DOI:10.1016/j.exphem.2009.02.010