Genome sequencing, assembly, and characterization of Pichia fermentans Z9Y-3 as a non-Saccharomyces yeast with aroma enhancing potential

• Published in: Food bioscience 2023-06, Vol.53, p.102701, Article 102701
• Main Authors: Fan, Tongtong, Qu, Jianing, Wang, Lu, Zhang, Jiarui, Yang, Xiaobing, Zhang, Hongyan, Qin, Yi, Tao, Yongsheng, Jin, Guojie
• Format: Article
• Language: English
• Subjects: Annotation; Aroma; Genome sequencing; Non-Saccharomyces yeast; Wine
• ISSN: 2212-4292; 2212-4306
• DOI: 10.1016/j.fbio.2023.102701

Pichia fermentans is an important non-Saccharomyces yeast applied in mixed fermentation to enhance fruity and floral traits of wine aroma. In this study, genome sequencing of P. fermentans Z9Y-3 was performed using the PacBio single-molecule real-time sequencing platform, and was further assembled using MECAT. The sequenced genes are annotated by non-redundant protein (Nr), Gene Ontology (GO), Cluster of Orthologous Groups of proteins (COG), Kyoto Encyclopedia of Genes and Genomes (KEGG) and carbohydrate-active enzymes (CAZymes) database. Results indicated that the genome size was about 13.5 Mb with 42.09% GC content and 4730 protein coding genes. The 95.90% genes were annotated to the non-redundant protein (Nr) database, and then annotated to GO database. The 62.64% genes annotated to the COG database revealed 25 functional groups. The 58.22% genes annotated to the KEGG database revealed 5 groups. The 586 genes were annotated to CAZymes, in which two genes bglH and bglC encoding beta-glucosidase were found and further analyzed its motifs. A terpene and a nonribosomal peptides like secondary metabolic gene clusters were predicted using antiSMASH 7.0.0. This study benefits us to understand physiological and biochemical behavior of P. fermentans Z9Y-3 at a molecular level to better use P. fermentans Z9Y-3 in aroma enhancing winemaking. •P. fermentans Z9Y-3 genome was about 13.5 Mb with 42.09% GC content.•P. fermentans Z9Y-3 genome includes 4730 genes coding proteins.•COG annotation of P. fermentans Z9Y-3 was different from that of other yeasts.•Genes bglH and bglC encoding beta-glucosidase were found in P. fermentans Z9Y-3.•Molecular basis of physio-biochemistry behavior benefits better use of P. fermentans.