Bio-imprinted lipase-catalyzed production of medium- and long-chain structured lipids rich in n-3 polyunsaturated fatty acids by acidolysis

• Published in: Food bioscience 2024-06, Vol.59, p.104025, Article 104025
• Main Authors: Zou, Xiaoqiang, Hussain, Mudassar, Khan, Imad, Wang, Yanxi, Jiang, Bangzhi, Zheng, Lei, Pan, Yuechao, Hu, Jijie, Ashraf, Azqa
• Format: Article
• Language: English
• Subjects: Acidolysis; anchovies; Bio-imprinting; carboxylic ester hydrolases; catalysts; Docosahexaenoic acid; Eicosapentaenoic acid; fish oils; Medium- and long-chain structured lipids; octanoic acid; sardines
• ISSN: 2212-4292
• DOI: 10.1016/j.fbio.2024.104025

Medium- and long-chain structured lipids (MLSLs) rich in docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA) were synthesized by acidolysis of fish oil (Omax 1812, a mixture of sardine and anchovy oils) with caprylic acid (CA) using a lipase bio-imprinted with fatty acids as a catalyst. A bio-imprinted lipase for the acidolysis reaction was first prepared under optimal conditions, and its activity was 1.43 times higher than that of the non-bioimprinted. The bio-imprinted lipase was used as a catalyst to produce such MLSLs, and the reaction conditions were optimized. Under the optimal conditions, equilibrium was achieved in 8 h, and the total and the sn-1, 3 CA contents in the obtained product were 28.72% and 42.26%. Additionally, the total and the sn-2 DHA contents were 14.23% and 21.10%, respectively, and the total and the sn-2 EPA contents were 16.43% and 10.14%, respectively. These obtained MLSLs, rich in sn-1, 3 CA, and sn-2 DHA, EPA can deliver significant health benefits. [Display omitted] •The lipase was bio-imprinted with fatty acids as a template.•The activity of bio-imprinted lipase was 1.43 times higher than that of the non-bioimprinted (control).•MLSLs synthesis by acidolysis of fish oil with caprylic acid (CA) using a bio-imprinted lipase as a catalyst.•The contents of sn-1, 3 CA, and sn-2 DHA, EPA in MLSLs were 42.26%, 21.10%, and 10.14%, respectively.•The obtained MLSLs, rich in CA, DHA, and EPA, can deliver significant health benefits.