Toxicity and carcinogenicity studies of methylene blue trihydrate in F344N rats and B6C3F1 mice

• Published in: Food and chemical toxicology 2010, Vol.48 (1), p.169-177
• Main Authors: Auerbach, Scott S., Bristol, Douglas W., Peckham, John C., Travlos, Gregory S., Hébert, Charles D., Chhabra, Rajendra S.
• Format: Article
• Language: English
• Subjects: adverse effects; animal models; Animals; Biological and medical sciences; Blood Cell Count; Body Weight - drug effects; Carcinogenesis, carcinogens and anticarcinogens; Carcinogenicity; Carcinogenicity Tests; Carcinogens - toxicity; Chemical agents; Chronic toxicity; dose response; Dose-Response Relationship, Drug; Female; Hematopoietic Stem Cells - drug effects; Hyperplasia - chemically induced; Hyperplasia - pathology; Kaplan-Meier Estimate; Lung - pathology; Male; Mammary Neoplasms, Experimental - chemically induced; Mammary Neoplasms, Experimental - pathology; Medical sciences; Methemoglobin; Methylene Blue - toxicity; Methylene blue trihydrate; Mice; Mice, Inbred Strains; neoplasms; Neoplasms - chemically induced; Neoplasms - pathology; oral administration; Rats; Rats, Inbred F344; Sex Characteristics; Species Specificity; Survival Analysis; toxicity; toxicity testing; Toxicology; Tumors
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2009.09.034

Methylene blue trihydrate has a variety of biomedical and biologically therapeutic applications. Groups of 50 male and 50 female rats and mice were administered methylene blue trihydrate in 0.5% aqueous methylcellulose solution by gavage at doses of 0, 5, 25, or 50 mg/kg bw/day (rats) or 0, 2.5, 12.5, and 25 mg/kg bw/day (mice), 5 days per week for 2 years. In rats survival of all dosed groups was similar to that of the vehicle controls, whereas mice exhibited a dose-dependent increase in survival. Rats receiving 25 and 50 mg/kg bw/day and mice receiving 25 mg/kg bw/day developed mild anemia. The incidences of pancreatic islet cell adenoma and adenoma or carcinoma (combined) were increased in all dosed groups of male rats, but increases were statistically significant in 25 mg/kg bw/day males only and the dose–response was non-linear. There was a corresponding increase in the incidence of pancreatic islet cell hyperplasia but statistically significant only in the 50 mg/kg bw/day male rats. There were no significant increases in neoplastic transformation observed in the mice; however, positive trends were noted for adenoma or carcinoma (combined) of the small intestine and malignant lymphoma.