Hepatoprotective effect of 10% ethanolic extract from Curdrania tricuspidata leaves against ethanol-induced oxidative stress through suppression of CYP2E1

• Published in: Food and chemical toxicology 2017-10, Vol.108 (Pt A), p.298-304
• Main Authors: You, Yanghee, Min, Seoyoung, Lee, Yoo-Hyun, Hwang, Kwontack, Jun, Woojin
• Format: Article
• Language: English
• Subjects: Animals; Chemical and Drug Induced Liver Injury - prevention & control; Curdrania tricuspidata; CYP2El; Cytochrome P-450 CYP2E1 - genetics; Cytochrome P-450 CYP2E1 - metabolism; Ethanol; Ethanol - toxicity; Gene Expression Regulation, Enzymologic - drug effects; Hep G2 Cells; Hepatoprotection; Humans; Maclura - chemistry; Male; Mice; Mice, Inbred C57BL; Oxidative stress; Oxidative Stress - drug effects; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2017.08.007

The hepatoprotective effect of 10% ethanolic extract of Curdrania tricuspidata (CTE) was investigated in HepG2/2E1 cells and C57BL/6 J mice. When compared ethanol-only treated HepG2/2E1 cells, pretreatment of CTE prevented increased intra-cellular reactive oxygen species levels and decreased antioxidant activities by ethanol-induced oxidative stress. In C57BL/6 J mice, CTE at a dose of 250 mg/kg/day was administered for 10 days, with ethanol (5 g/kg/day) administered for the final 3 days. Pretreatment with CTE prevented the elevated activities of serum aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase caused by ethanol-induced hepatic damage. CTE-treated mice displayed a reduced level of malondialdehyde and increased antioxidant activities of catalase, glutathione S-transferase, glutathione peroxidase, and superoxide dismutase, as well as a reduced level of glutathione as compared with ethanol-only-treated mice. CTE-treated mice exhibited significant inhibition of CYP2E1 activities and expression. These results suggest that CTE could be a useful agent for the prevention of ethanol-induced oxidative damage in the liver, elevating antioxidative potentials and alleviating oxidative stress by suppressing CYP2El. [Display omitted] •10% ethanolic extract of C. tricuspidata leaves reduced ethanol-induced hepatic damages.•10% ethanolic extract of C. tricuspidata leaves elevated antioxidant defense capacity.•10% ethanolic extract of C. tricuspidata leaves suppressed CYP2E1 activity and protein expression.