Loading…

Altered paracellular permeability in intestinal cell monolayer challenged with lipopolysaccharide: Modulatory effects of pterostilbene metabolites

Epithelial barrier alteration is a central event in the pathogenesis of inflammatory bowel diseases. Lipopolysaccharide, correlated to the pathogenesis of such pathologies, has been demonstrated to cause altered membrane permeability, through the disruption and/or relocation of tight junction protei...

Full description

Saved in:
Bibliographic Details
Published in:Food and chemical toxicology 2020-11, Vol.145, p.111729, Article 111729
Main Authors: Serreli, Gabriele, Melis, Maria Paola, Zodio, Sonia, Naitza, Micaela Rita, Casula, Emanuela, Peñalver, Pablo, Lucas, Ricardo, Loi, Roberto, Morales, Juan Carlos, Deiana, Monica
Format: Article
Language:English
Subjects:
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c353t-a06d6a6fe77998caafc2da84c91f202104ef1a66bffbf3b7683284a7089491d73
cites cdi_FETCH-LOGICAL-c353t-a06d6a6fe77998caafc2da84c91f202104ef1a66bffbf3b7683284a7089491d73
container_end_page
container_issue
container_start_page 111729
container_title Food and chemical toxicology
container_volume 145
creator Serreli, Gabriele
Melis, Maria Paola
Zodio, Sonia
Naitza, Micaela Rita
Casula, Emanuela
Peñalver, Pablo
Lucas, Ricardo
Loi, Roberto
Morales, Juan Carlos
Deiana, Monica
description Epithelial barrier alteration is a central event in the pathogenesis of inflammatory bowel diseases. Lipopolysaccharide, correlated to the pathogenesis of such pathologies, has been demonstrated to cause altered membrane permeability, through the disruption and/or relocation of tight junction proteins, following redox-sensitive mitogen-activated protein kinases (MAPKs) modulation. Pterostilbene and its metabolite pinostilbene are natural stilbenoids which may reach relevant concentrations at intestinal level, together with their glucuronide and sulfate metabolites. The aim of our study was to evaluate the ability of these compounds to inhibit lipopolysaccharide-induced toxic effects on intestinal cell monolayer integrity and to explore the mechanism of action. Caco-2 cells, differentiated as enterocytes, were treated with lipopolysaccharide following pretreatment with the phenolic compounds at 1 μM physiological concentration. Caco-2 monolayer's permeability was monitored with time, measuring the transepithelial electrical resistance. Tight junction proteins were assessed by western blotting and immunofluorescence in lipopolysaccharide-treated cells, in relation to MAPK p38 and ERK1/2 activation. Pretreatment with all the phenolic compounds significantly slowed lipopolysaccharide-induced transepithelial electrical resistance decrease, preserved tight junction proteins levels and reduced MAPKs phosphorylation. The reported findings indicate that pterostilbene and its metabolites may counteract lipopolysaccharide-induced alteration of epithelial permeability, one of the initial events in the intestinal inflammatory process. [Display omitted] •Pinostilbene, pterostilbene and metabolites were tested in LPS-treated Caco-2 cells.•LPS induced alteration of monolayer permeability through tight junctions disruption.•Metabolites and parent compounds inhibited LPS toxic effects to the same extent.•These effects depend on the modulation of MAPK p38 and ERK1/2 phosphorylation.
doi_str_mv 10.1016/j.fct.2020.111729
format article
fullrecord <record><control><sourceid>pubmed_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1016_j_fct_2020_111729</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0278691520306190</els_id><sourcerecordid>32898597</sourcerecordid><originalsourceid>FETCH-LOGICAL-c353t-a06d6a6fe77998caafc2da84c91f202104ef1a66bffbf3b7683284a7089491d73</originalsourceid><addsrcrecordid>eNp9kMFO3DAQhq2qqCzQB-il8gtkaye7dtyeEAJaaSsucLYm9rh45cSRbajyGjwxXm3LEcnSyJr__2fmI-QLZ2vOuPi2XztT1i1r659z2aoPZMV72TWi2_KPZMVa2TdC8e0pOct5zxiTXIpP5LRre9VvlVyRl8tQMKGlMyQwGMJTgERnTCPC4IMvC_VTfQVz8RMEetDQMU4xwIKJmkcIAac_NeGvL480-DnOMSwZTG0lb_E7_R1tTS0xLRSdQ1MyjY7OdW6soWHACemIBYZY52G-ICcOQsbP_-o5ebi5vr_62ezubn9dXe4a02270gATVoBwKKVSvQFwprXQb4ziriLhbIOOgxCDc4PrBin6evUGJOvVRnEru3PCj7mm7pETOj0nP0JaNGf6wFfvdeWrD3z1kW_1fD165qdhRPvm-A-0Cn4cBVg3f_aYdDYeJ4PWp3q5ttG_E_8Kc6-QkQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Altered paracellular permeability in intestinal cell monolayer challenged with lipopolysaccharide: Modulatory effects of pterostilbene metabolites</title><source>ScienceDirect Freedom Collection 2022-2024</source><creator>Serreli, Gabriele ; Melis, Maria Paola ; Zodio, Sonia ; Naitza, Micaela Rita ; Casula, Emanuela ; Peñalver, Pablo ; Lucas, Ricardo ; Loi, Roberto ; Morales, Juan Carlos ; Deiana, Monica</creator><creatorcontrib>Serreli, Gabriele ; Melis, Maria Paola ; Zodio, Sonia ; Naitza, Micaela Rita ; Casula, Emanuela ; Peñalver, Pablo ; Lucas, Ricardo ; Loi, Roberto ; Morales, Juan Carlos ; Deiana, Monica</creatorcontrib><description>Epithelial barrier alteration is a central event in the pathogenesis of inflammatory bowel diseases. Lipopolysaccharide, correlated to the pathogenesis of such pathologies, has been demonstrated to cause altered membrane permeability, through the disruption and/or relocation of tight junction proteins, following redox-sensitive mitogen-activated protein kinases (MAPKs) modulation. Pterostilbene and its metabolite pinostilbene are natural stilbenoids which may reach relevant concentrations at intestinal level, together with their glucuronide and sulfate metabolites. The aim of our study was to evaluate the ability of these compounds to inhibit lipopolysaccharide-induced toxic effects on intestinal cell monolayer integrity and to explore the mechanism of action. Caco-2 cells, differentiated as enterocytes, were treated with lipopolysaccharide following pretreatment with the phenolic compounds at 1 μM physiological concentration. Caco-2 monolayer's permeability was monitored with time, measuring the transepithelial electrical resistance. Tight junction proteins were assessed by western blotting and immunofluorescence in lipopolysaccharide-treated cells, in relation to MAPK p38 and ERK1/2 activation. Pretreatment with all the phenolic compounds significantly slowed lipopolysaccharide-induced transepithelial electrical resistance decrease, preserved tight junction proteins levels and reduced MAPKs phosphorylation. The reported findings indicate that pterostilbene and its metabolites may counteract lipopolysaccharide-induced alteration of epithelial permeability, one of the initial events in the intestinal inflammatory process. [Display omitted] •Pinostilbene, pterostilbene and metabolites were tested in LPS-treated Caco-2 cells.•LPS induced alteration of monolayer permeability through tight junctions disruption.•Metabolites and parent compounds inhibited LPS toxic effects to the same extent.•These effects depend on the modulation of MAPK p38 and ERK1/2 phosphorylation.</description><identifier>ISSN: 0278-6915</identifier><identifier>EISSN: 1873-6351</identifier><identifier>DOI: 10.1016/j.fct.2020.111729</identifier><identifier>PMID: 32898597</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Caco-2 Cells ; Epithelial Cells - drug effects ; Epithelial Cells - metabolism ; Humans ; Inflammation ; Intestinal Mucosa - drug effects ; Intestinal Mucosa - metabolism ; Intestinal permeability ; Lipopolysaccharide ; Lipopolysaccharides - toxicity ; Metabolites ; Mitogen-Activated Protein Kinase 1 - genetics ; Mitogen-Activated Protein Kinase 1 - metabolism ; Mitogen-Activated Protein Kinase 3 - genetics ; Mitogen-Activated Protein Kinase 3 - metabolism ; p38 Mitogen-Activated Protein Kinases - genetics ; p38 Mitogen-Activated Protein Kinases - metabolism ; Permeability - drug effects ; Pterostilbene ; Stilbenes - metabolism ; Stilbenes - pharmacology ; Tight junctions</subject><ispartof>Food and chemical toxicology, 2020-11, Vol.145, p.111729, Article 111729</ispartof><rights>2020 Elsevier Ltd</rights><rights>Copyright © 2020 Elsevier Ltd. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c353t-a06d6a6fe77998caafc2da84c91f202104ef1a66bffbf3b7683284a7089491d73</citedby><cites>FETCH-LOGICAL-c353t-a06d6a6fe77998caafc2da84c91f202104ef1a66bffbf3b7683284a7089491d73</cites><orcidid>0000-0003-0259-6103 ; 0000-0002-7166-1856</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32898597$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Serreli, Gabriele</creatorcontrib><creatorcontrib>Melis, Maria Paola</creatorcontrib><creatorcontrib>Zodio, Sonia</creatorcontrib><creatorcontrib>Naitza, Micaela Rita</creatorcontrib><creatorcontrib>Casula, Emanuela</creatorcontrib><creatorcontrib>Peñalver, Pablo</creatorcontrib><creatorcontrib>Lucas, Ricardo</creatorcontrib><creatorcontrib>Loi, Roberto</creatorcontrib><creatorcontrib>Morales, Juan Carlos</creatorcontrib><creatorcontrib>Deiana, Monica</creatorcontrib><title>Altered paracellular permeability in intestinal cell monolayer challenged with lipopolysaccharide: Modulatory effects of pterostilbene metabolites</title><title>Food and chemical toxicology</title><addtitle>Food Chem Toxicol</addtitle><description>Epithelial barrier alteration is a central event in the pathogenesis of inflammatory bowel diseases. Lipopolysaccharide, correlated to the pathogenesis of such pathologies, has been demonstrated to cause altered membrane permeability, through the disruption and/or relocation of tight junction proteins, following redox-sensitive mitogen-activated protein kinases (MAPKs) modulation. Pterostilbene and its metabolite pinostilbene are natural stilbenoids which may reach relevant concentrations at intestinal level, together with their glucuronide and sulfate metabolites. The aim of our study was to evaluate the ability of these compounds to inhibit lipopolysaccharide-induced toxic effects on intestinal cell monolayer integrity and to explore the mechanism of action. Caco-2 cells, differentiated as enterocytes, were treated with lipopolysaccharide following pretreatment with the phenolic compounds at 1 μM physiological concentration. Caco-2 monolayer's permeability was monitored with time, measuring the transepithelial electrical resistance. Tight junction proteins were assessed by western blotting and immunofluorescence in lipopolysaccharide-treated cells, in relation to MAPK p38 and ERK1/2 activation. Pretreatment with all the phenolic compounds significantly slowed lipopolysaccharide-induced transepithelial electrical resistance decrease, preserved tight junction proteins levels and reduced MAPKs phosphorylation. The reported findings indicate that pterostilbene and its metabolites may counteract lipopolysaccharide-induced alteration of epithelial permeability, one of the initial events in the intestinal inflammatory process. [Display omitted] •Pinostilbene, pterostilbene and metabolites were tested in LPS-treated Caco-2 cells.•LPS induced alteration of monolayer permeability through tight junctions disruption.•Metabolites and parent compounds inhibited LPS toxic effects to the same extent.•These effects depend on the modulation of MAPK p38 and ERK1/2 phosphorylation.</description><subject>Caco-2 Cells</subject><subject>Epithelial Cells - drug effects</subject><subject>Epithelial Cells - metabolism</subject><subject>Humans</subject><subject>Inflammation</subject><subject>Intestinal Mucosa - drug effects</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestinal permeability</subject><subject>Lipopolysaccharide</subject><subject>Lipopolysaccharides - toxicity</subject><subject>Metabolites</subject><subject>Mitogen-Activated Protein Kinase 1 - genetics</subject><subject>Mitogen-Activated Protein Kinase 1 - metabolism</subject><subject>Mitogen-Activated Protein Kinase 3 - genetics</subject><subject>Mitogen-Activated Protein Kinase 3 - metabolism</subject><subject>p38 Mitogen-Activated Protein Kinases - genetics</subject><subject>p38 Mitogen-Activated Protein Kinases - metabolism</subject><subject>Permeability - drug effects</subject><subject>Pterostilbene</subject><subject>Stilbenes - metabolism</subject><subject>Stilbenes - pharmacology</subject><subject>Tight junctions</subject><issn>0278-6915</issn><issn>1873-6351</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNp9kMFO3DAQhq2qqCzQB-il8gtkaye7dtyeEAJaaSsucLYm9rh45cSRbajyGjwxXm3LEcnSyJr__2fmI-QLZ2vOuPi2XztT1i1r659z2aoPZMV72TWi2_KPZMVa2TdC8e0pOct5zxiTXIpP5LRre9VvlVyRl8tQMKGlMyQwGMJTgERnTCPC4IMvC_VTfQVz8RMEetDQMU4xwIKJmkcIAac_NeGvL480-DnOMSwZTG0lb_E7_R1tTS0xLRSdQ1MyjY7OdW6soWHACemIBYZY52G-ICcOQsbP_-o5ebi5vr_62ezubn9dXe4a02270gATVoBwKKVSvQFwprXQb4ziriLhbIOOgxCDc4PrBin6evUGJOvVRnEru3PCj7mm7pETOj0nP0JaNGf6wFfvdeWrD3z1kW_1fD165qdhRPvm-A-0Cn4cBVg3f_aYdDYeJ4PWp3q5ttG_E_8Kc6-QkQ</recordid><startdate>202011</startdate><enddate>202011</enddate><creator>Serreli, Gabriele</creator><creator>Melis, Maria Paola</creator><creator>Zodio, Sonia</creator><creator>Naitza, Micaela Rita</creator><creator>Casula, Emanuela</creator><creator>Peñalver, Pablo</creator><creator>Lucas, Ricardo</creator><creator>Loi, Roberto</creator><creator>Morales, Juan Carlos</creator><creator>Deiana, Monica</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0003-0259-6103</orcidid><orcidid>https://orcid.org/0000-0002-7166-1856</orcidid></search><sort><creationdate>202011</creationdate><title>Altered paracellular permeability in intestinal cell monolayer challenged with lipopolysaccharide: Modulatory effects of pterostilbene metabolites</title><author>Serreli, Gabriele ; Melis, Maria Paola ; Zodio, Sonia ; Naitza, Micaela Rita ; Casula, Emanuela ; Peñalver, Pablo ; Lucas, Ricardo ; Loi, Roberto ; Morales, Juan Carlos ; Deiana, Monica</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c353t-a06d6a6fe77998caafc2da84c91f202104ef1a66bffbf3b7683284a7089491d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Caco-2 Cells</topic><topic>Epithelial Cells - drug effects</topic><topic>Epithelial Cells - metabolism</topic><topic>Humans</topic><topic>Inflammation</topic><topic>Intestinal Mucosa - drug effects</topic><topic>Intestinal Mucosa - metabolism</topic><topic>Intestinal permeability</topic><topic>Lipopolysaccharide</topic><topic>Lipopolysaccharides - toxicity</topic><topic>Metabolites</topic><topic>Mitogen-Activated Protein Kinase 1 - genetics</topic><topic>Mitogen-Activated Protein Kinase 1 - metabolism</topic><topic>Mitogen-Activated Protein Kinase 3 - genetics</topic><topic>Mitogen-Activated Protein Kinase 3 - metabolism</topic><topic>p38 Mitogen-Activated Protein Kinases - genetics</topic><topic>p38 Mitogen-Activated Protein Kinases - metabolism</topic><topic>Permeability - drug effects</topic><topic>Pterostilbene</topic><topic>Stilbenes - metabolism</topic><topic>Stilbenes - pharmacology</topic><topic>Tight junctions</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Serreli, Gabriele</creatorcontrib><creatorcontrib>Melis, Maria Paola</creatorcontrib><creatorcontrib>Zodio, Sonia</creatorcontrib><creatorcontrib>Naitza, Micaela Rita</creatorcontrib><creatorcontrib>Casula, Emanuela</creatorcontrib><creatorcontrib>Peñalver, Pablo</creatorcontrib><creatorcontrib>Lucas, Ricardo</creatorcontrib><creatorcontrib>Loi, Roberto</creatorcontrib><creatorcontrib>Morales, Juan Carlos</creatorcontrib><creatorcontrib>Deiana, Monica</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><jtitle>Food and chemical toxicology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Serreli, Gabriele</au><au>Melis, Maria Paola</au><au>Zodio, Sonia</au><au>Naitza, Micaela Rita</au><au>Casula, Emanuela</au><au>Peñalver, Pablo</au><au>Lucas, Ricardo</au><au>Loi, Roberto</au><au>Morales, Juan Carlos</au><au>Deiana, Monica</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Altered paracellular permeability in intestinal cell monolayer challenged with lipopolysaccharide: Modulatory effects of pterostilbene metabolites</atitle><jtitle>Food and chemical toxicology</jtitle><addtitle>Food Chem Toxicol</addtitle><date>2020-11</date><risdate>2020</risdate><volume>145</volume><spage>111729</spage><pages>111729-</pages><artnum>111729</artnum><issn>0278-6915</issn><eissn>1873-6351</eissn><abstract>Epithelial barrier alteration is a central event in the pathogenesis of inflammatory bowel diseases. Lipopolysaccharide, correlated to the pathogenesis of such pathologies, has been demonstrated to cause altered membrane permeability, through the disruption and/or relocation of tight junction proteins, following redox-sensitive mitogen-activated protein kinases (MAPKs) modulation. Pterostilbene and its metabolite pinostilbene are natural stilbenoids which may reach relevant concentrations at intestinal level, together with their glucuronide and sulfate metabolites. The aim of our study was to evaluate the ability of these compounds to inhibit lipopolysaccharide-induced toxic effects on intestinal cell monolayer integrity and to explore the mechanism of action. Caco-2 cells, differentiated as enterocytes, were treated with lipopolysaccharide following pretreatment with the phenolic compounds at 1 μM physiological concentration. Caco-2 monolayer's permeability was monitored with time, measuring the transepithelial electrical resistance. Tight junction proteins were assessed by western blotting and immunofluorescence in lipopolysaccharide-treated cells, in relation to MAPK p38 and ERK1/2 activation. Pretreatment with all the phenolic compounds significantly slowed lipopolysaccharide-induced transepithelial electrical resistance decrease, preserved tight junction proteins levels and reduced MAPKs phosphorylation. The reported findings indicate that pterostilbene and its metabolites may counteract lipopolysaccharide-induced alteration of epithelial permeability, one of the initial events in the intestinal inflammatory process. [Display omitted] •Pinostilbene, pterostilbene and metabolites were tested in LPS-treated Caco-2 cells.•LPS induced alteration of monolayer permeability through tight junctions disruption.•Metabolites and parent compounds inhibited LPS toxic effects to the same extent.•These effects depend on the modulation of MAPK p38 and ERK1/2 phosphorylation.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>32898597</pmid><doi>10.1016/j.fct.2020.111729</doi><orcidid>https://orcid.org/0000-0003-0259-6103</orcidid><orcidid>https://orcid.org/0000-0002-7166-1856</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 0278-6915
ispartof Food and chemical toxicology, 2020-11, Vol.145, p.111729, Article 111729
issn 0278-6915
1873-6351
language eng
recordid cdi_crossref_primary_10_1016_j_fct_2020_111729
source ScienceDirect Freedom Collection 2022-2024
subjects Caco-2 Cells
Epithelial Cells - drug effects
Epithelial Cells - metabolism
Humans
Inflammation
Intestinal Mucosa - drug effects
Intestinal Mucosa - metabolism
Intestinal permeability
Lipopolysaccharide
Lipopolysaccharides - toxicity
Metabolites
Mitogen-Activated Protein Kinase 1 - genetics
Mitogen-Activated Protein Kinase 1 - metabolism
Mitogen-Activated Protein Kinase 3 - genetics
Mitogen-Activated Protein Kinase 3 - metabolism
p38 Mitogen-Activated Protein Kinases - genetics
p38 Mitogen-Activated Protein Kinases - metabolism
Permeability - drug effects
Pterostilbene
Stilbenes - metabolism
Stilbenes - pharmacology
Tight junctions
title Altered paracellular permeability in intestinal cell monolayer challenged with lipopolysaccharide: Modulatory effects of pterostilbene metabolites
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T17%3A15%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Altered%20paracellular%20permeability%20in%20intestinal%20cell%20monolayer%20challenged%20with%20lipopolysaccharide:%20Modulatory%20effects%20of%20pterostilbene%20metabolites&rft.jtitle=Food%20and%20chemical%20toxicology&rft.au=Serreli,%20Gabriele&rft.date=2020-11&rft.volume=145&rft.spage=111729&rft.pages=111729-&rft.artnum=111729&rft.issn=0278-6915&rft.eissn=1873-6351&rft_id=info:doi/10.1016/j.fct.2020.111729&rft_dat=%3Cpubmed_cross%3E32898597%3C/pubmed_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c353t-a06d6a6fe77998caafc2da84c91f202104ef1a66bffbf3b7683284a7089491d73%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/32898597&rfr_iscdi=true