Ellagitannins from jabuticaba (Myrciaria jaboticaba) seeds attenuated inflammation, oxidative stress, aberrant crypt foci, and modulated gut microbiota in rats with 1,2 dimethyl hydrazine-induced colon carcinogenesis

• Published in: Food and chemical toxicology 2021-08, Vol.154, p.112287, Article 112287
• Main Authors: do Carmo, Mariana Araújo Vieira, Fidelis, Marina, de Oliveira, Pollyanna Francielli, Feitoza, Lais Quellen, Marques, Marcos José, Ferreira, Eric Batista, Oh, Won Young, Shahidi, Fereidoon, Hellström, Jarkko, Almeida, Leonardo Augusto, Novaes, Rômulo Dias, Granato, Daniel, Azevedo, Luciana
• Format: Article
• Language: English
• Subjects: Aberrant crypt; Cancer; DMH; Microbiota; Myrciaria jaboticaba; Phenolic compounds
• ISSN: 0278-6915; 1873-6351
• DOI: 10.1016/j.fct.2021.112287

Since dietary factors are thought to be responsible for high colon cancer risk, we investigated the chemopreventive effect of jabuticaba seed extract (LJE) by administering yogurt with or without LJE against 1,2 dimethyl hydrazine (DMH)-induced colon carcinogenesis in rats. Results showed that LJE contained a total phenolic content of 57.16 g/100 g of seed extract in which 7.67 and 10.09 g/100 g represented total flavonoids and ellagitannins, respectively. LJE protected DNA and human LDL against induced in vitro oxidation, which was associated with the ellagitannin content and with the free-radical scavenging and reducing capacities. LJE alone had a non-clastogenicity/aneugenicity property, but in combination with cisplatin, it enhanced the chromosome aberrations in cancer cells. In colon cancer-induced rats, yogurt with or without LJE caused a reduction in pro-inflammatory parameters, decreased the RNA expression of antiapoptotic cytokines and increased the expression of proapoptotic cytokines. Moreover, LJE attenuated colon cancer initiation and progression by decreasing aberrant crypt foci and LJE recovered the gut microbiome. Together, this evidence suggests that LJE provides chemopreventive protection against colon cancer development by reducing inflammation and increasing proapoptotic pathways. [Display omitted] •Jabuticaba seed extract (LJE) is a rich source of phenolic compounds with high antioxidant activity.•LJE attenuated the expression of pro-inflammatory cytokines, COX-2, LDL oxidation and DNA damage.•Yogurt with or without LJE reduced colon aberrant crypt foci incidence.•LJE exerted anticancer role via induction of apoptosis.•Yogurt with or without LJE modulated the gut microbiota in 1,2-dimethylhydrazine-induced colon cancer rats.