Point mutations in the barley HvHAK1 potassium transporter lead to improved K +-nutrition and enhanced resistance to salt stress

Members of group I KT-HAK-KUP transporters play an important role in K + acquisition by plant roots, a process that is strongly affected by salt stress. A PCR-based random mutagenesis approach on HvHAK1 allowed identification of V366I and R591C substitutions, which confer enhanced K +-capture, and i...

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Bibliographic Details
Published in:FEBS letters 2008-11, Vol.582 (28), p.3922-3928
Main Authors: Mangano, Silvina, Silberstein, Susana, Santa-María, Guillermo E.
Format: Article
Language:English
Subjects:
Amino Acid Substitution
Arginine - genetics
Arginine - metabolism
Cation Transport Proteins - chemistry
Cation Transport Proteins - genetics
Cation Transport Proteins - metabolism
Cysteine - genetics
Cysteine - metabolism
HAK
Ion Transport
Isoleucine - genetics
Isoleucine - metabolism
KUP
Osmotic Pressure
Plant Proteins - chemistry
Plant Proteins - genetics
Plant Proteins - metabolism
Point Mutation
Potassium
Potassium - metabolism
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins - genetics
Salinity
Sodium
Sodium - metabolism
Sodium Chloride - metabolism
Transporter
Valine - genetics
Valine - metabolism
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Summary:Members of group I KT-HAK-KUP transporters play an important role in K + acquisition by plant roots, a process that is strongly affected by salt stress. A PCR-based random mutagenesis approach on HvHAK1 allowed identification of V366I and R591C substitutions, which confer enhanced K +-capture, and improved NaCl, LiCl and NH 4Cl tolerance, to yeast cells. Improved K +-capture was linked to an enhanced V max. Results reveal an intrinsic protective effect of K +, and assign an important role to the 8th transmembrane domain, as well as the C-terminus, in determining the maximum capacity for the transport of K + in KT-HAK-KUP transporters.
ISSN:0014-5793
1873-3468
DOI:10.1016/j.febslet.2008.10.036