Dynamin-1 co-associates with native mouse brain BK Ca channels: Proteomics analysis of synaptic protein complexes

• Published in: FEBS letters 2010-03, Vol.584 (5), p.845-851
• Main Authors: Gorini, Giorgio, Ponomareva, Olga, Shores, Kevin S., Person, Maria D., Harris, R. Adron, Mayfield, R. Dayne
• Format: Article
• Language: English
• Subjects: Exocytosis; Immunoprecipitation; Mass spectrometry; Protein–protein interaction; Soluble N-ethylmaleimide-sensitive factor attachment protein receptor; Synaptic vesicle
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2009.12.061

In every synapse, a large number of proteins interact with other proteins in order to carry out signaling and transmission in the central nervous system. In this study, we used interaction proteomics to identify novel synaptic protein interactions in mouse cortical membranes under native conditions. Using immunoprecipitation, immunoblotting, and mass spectrometry, we identified a number of novel synaptic protein interactions involving soluble N-ethylmaleimide-sensitive factor attachment protein receptors (SNAREs), calcium-activated potassium channel (BK Ca) alpha subunits, and dynamin-1. These novel interactions offer valuable insight into the protein–protein interaction network in intact synapses that could advance understanding of vesicle trafficking, release, and recycling. MINT- 7543319: Snap-25 (uniprotkb: P60879) physically interacts (MI: 0914) with Tubulin beta-5 chain (uniprotkb: P99024), V-type proton ATPase subunit d 1 (uniprotkb: P51863), Zinc finger homeobox protein 3 (uniprotkb: Q61329), Tubulin beta-2A chain (uniprotkb: Q7TMM9), Synaptophysin (uniprotkb: Q62277), Gapdh (uniprotkb: P16858), Basement membrane-specific heparan sulfate proteoglycan core protein (uniprotkb: Q05793), Tubulin alpha-4A chain (uniprotkb: P68368), Tubulin alpha-1A chain (uniprotkb: P68369), Microtubule-associated protein 6 (uniprotkb: Q7TSJ2), AP-2 complex subunit beta (uniprotkb: Q9DBG3), Phosphofurin acidic cluster sorting protein 1 (uniprotkb: Q8K212), AP-2 complex subunit alpha-1 (uniprotkb: P17426), Kinesin-1 heavy chain (uniprotkb: Q617r68), Kinesin heavy chain isoform 5C (uniprotkb: P28738), Sodium/potassium-transporting ATPase subunit alpha-1 (uniprotkb: Q8VDN2) and Nck-associated protein 1 (uniprotkb: P28660) by anti bait co-immunoprecipitation (MI: 0006) MINT- 7543636: Calcium-activated potassium channel subunit alpha-1 (uniprotkb: Q08460) physically interacts (MI: 0914) with AMP deaminase 2 (uniprotkb: Q9DBT5), Gamma-tubulin complex component 4 (uniprotkb: Q9D4F8), Gamma-tubulin complex component 2 (uniprotkb: Q921G8), Sodium/potassium-transporting ATPase subunit alpha-1 (uniprotkb: Q8VDN2), Phosphoinositide 3-kinase regulatory subunit 4 (uniprotkb: Q8VD65), Beta-centractin (uniprotkb: Q8R5C5), KIAA1107 (uniprotkb: Q80TK0), Sodium/potassium-transporting ATPase subunit alpha-2 (uniprotkb: Q6PIE5), Sodium/potassium-transporting ATPase subunit alpha-3 (uniprotkb: Q6PIC6), Phosphatidylinositol 3-kinase catalytic subunit type 3 (uniprotkb: Q6PF93), KH domain-cont