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Experimental evidence suggesting that H 2 O 2 is produced within the thylakoid membrane in a reaction between plastoquinol and singlet oxygen
Plastoquinol (PQH 2 ‐9) and plastoquinone (PQ‐9) mediate photosynthetic electron transfer. We isolated PQH 2 ‐9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH 2 ‐9 to singlet oxygen ( 1 O 2 ) and analyzed the products. The main reaction of 1 O 2 with PQH 2 ‐9 in methanol...
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| Published in: | FEBS letters 2015-03, Vol.589 (6), p.779-786 |
|---|---|
| Main Authors: | , , |
| Format: | Article |
| Language: | English |
| Citations: | Items that this one cites Items that cite this one |
| Online Access: | Get full text |
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| Summary: | Plastoquinol (PQH
2
‐9) and plastoquinone (PQ‐9) mediate photosynthetic electron transfer. We isolated PQH
2
‐9 from thylakoid membranes, purified it with HPLC, subjected the purified PQH
2
‐9 to singlet oxygen (
1
O
2
) and analyzed the products. The main reaction of
1
O
2
with PQH
2
‐9 in methanol was found to result in formation of PQ‐9 and H
2
O
2
, and the amount of H
2
O
2
produced was essentially the same as the amount of oxidized PQH
2
‐9. Formation of H
2
O
2
in the reaction between
1
O
2
and PQH
2
‐9 may be an important source of H
2
O
2
within the lipophilic thylakoid membrane.
Singlet oxygen oxidizes plastoquinol to plastoquinone.
Oxidation of plastoquinol by singlet oxygen produces hydrogen peroxide.
Hydrogen peroxide formation is stoichiometric with oxidation of plastoquinol.
Plastohydroquinone enhances the chemiluminescence of the luminol‐peroxidase system. |
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| ISSN: | 0014-5793 1873-3468 |
| DOI: | 10.1016/j.febslet.2015.02.011 |