Optimization of the selective preparation of 20(R)-ginsenoside Rg3 catalyzed by d, l-tartaric acid using response surface methodology

• Published in: Fitoterapia 2013-01, Vol.84, p.213-221
• Main Authors: Sun, Chengpeng, Gao, Weiping, Zhao, Baozhong, Cheng, Leqin
• Format: Article
• Language: English
• Subjects: 20(R)-ginsenoside Rg3; Hydrolysis; Protopanaxadiol type saponins; Response surface methodology; saponins; Selective preparation; temperature
• ISSN: 0367-326X
• DOI: 10.1016/j.fitote.2012.11.011

The optimization of the selective preparation of 20(R)-ginsenoside Rg3 converting protopanaxadiol type saponins (PPD saponins) by the commercially available d, l-tartaric acid was carried out using response surface methodology (RSM) based on a three-factor and six-level central composite design. The optimal 20(R)-ginsenoside Rg3 de% was predicted to be 94.52% in the combination of the factors (d, l-tartaric acid concentration 1.19mol/L, temperature 107.9°C and time 2.79h) through the canonical analysis with maximum responses. Under the optimum reaction conditions, the actual 20(R)-ginsenoside Rg3 de% was 96.49%. 20(R)-ginsenoside Rg3 (1) and 20(S)-ginsenoside Rg3 (2) were separated and identified by 1H-NMR and 13C-NMR. Therefore, the RSM was effective to optimize the preparation of 20(R)-ginsenoside Rg3 by converting PPD saponins using d, l-tartaric acid. 20(R)-ginsenoside Rg3 was selectively prepared by d, l-tartaric acid as the catalyst, and optimized by response surface methodology. [Display omitted]