The pharmacokinetic study on the mechanism of toxicity attenuation of rhubarb total free anthraquinone oral colon-specific drug delivery system

• Published in: Fitoterapia 2015-07, Vol.104, p.86-96
• Main Authors: Zhang, Lin, Chang, Jin-hua, Zhang, Bao-qi, Liu, Xi-gang, Liu, Pei, Xue, He-fei, Liu, Li-yan, Fu, Qiang, Zhu, Meng, Liu, Cui-zhe
• Format: Article
• Language: English
• Subjects: Oral colon-specific drug delivery system; Pharmacokinetics; Rhubarb anthraquinones; Toxicity attenuation
• ISSN: 0367-326X
• DOI: 10.1016/j.fitote.2015.05.018

Rhubarb is commonly used as laxatives in Asian countries, of which anthraquinones are the major active ingredients, but there are an increased number of concerns regarding the nephrotoxicity of anthraquinones. In this study, we compared the pharmacokinetic characteristics of rhubarb anthraquinones in rats after orally administered with rhubarb and rhubarb total free anthraquinone oral colon-specific drug delivery granules (RTFA-OCDD-GN), and then explained why these granules could reduce the nephrotoxicity of anthraquinones when they produced purgative efficacy. A sensitive and reliable high performance liquid chromatography (HPLC) method has been fully validated for simultaneous determination of the five active components of rhubarb, and successfully applied to investigate and compare the remarkable differences in pharmacokinetic study of rhubarb anthraquinones after orally administered with rhubarb and RTFA-OCDD-GN. The results showed that, compared with rhubarb group, the AUC, Cmax, t1/2z and Vz/F of aloe-emodin, rhein, emodin and chrysophanol in rats receiving the RTFA-OCDD-GN were significantly decreased, and the Tmax of the four analytes was prolonged. Moreover, the Tmax of rhein, the Cmax of chrysophanol and emodin all have significant differences (P