Euphopias G − J, macrocyclic diterpenes with anti-zika virus activity from Euphorbia helioscopia L

• Published in: Fitoterapia 2023-09, Vol.169, p.105614, Article 105614
• Main Authors: Qiu, Xiong, Jiang, Ying-Jie, Huang, Yong-Xiang, Pang, Wen-Hui, Wu, Ze-Kai, Zhou, Ya-Ling, Li, Rui, Bi, De-Wen, Cheng, Bin, Xiao, Wei-Lie, Zheng, Chang-Bo, Li, Xiao-Li
• Format: Article
• Language: English
• Subjects: Anti-Zika virus activity; Cytotoxicity; Diterpenoids; Euphorbia helioscopia L
• ISSN: 0367-326X
• DOI: 10.1016/j.fitote.2023.105614

Four new diterpenoids (1–4) and sixteen known diterpenoids (5–20) were purified from the whole plant of Euphorbia helioscopia L. Compounds 1 and 2 were rhamofolane diterpenoids with a 5/7/6 tricyclic systems, compound 3 was a lathyranes diterpenoid, and compound 4 was a jathophanes diterpenoid. The isolated compounds were tested for their cytotoxicity and anti-Zika virus properties, and compounds 9 and 15 showed low cytotoxicity and strong anti-Zika virus properties with EC50 2.63 and 5.94 μM, respectively. Further, the inhibitory effects of compounds on protein levels were determined using Western blotting and immunofluorescence assays. [Display omitted] •Twenty natural compounds were isolated from the Euphorbia helioscopia, of which four were new compounds.•Compounds 9 and 15 showed strong activity with EC50 values of 2.63 and 5.94 μM, respectively. In addition, the results of cytotoxicity tests showed that compounds 9 and 15 had relative cell activity of over 50% at 200 μM.