A simple, fast and reliable methodology for the analysis of histidine dipeptides as markers of the presence of animal origin proteins in feeds for ruminants

A simple methodology, consisting in cation exchange HPLC separation combined with OPA postcolumn detection, has been succesfully applied to the analysis and detection of histidine dipeptides in feeds. The use of the proposed methodology, which is very simple and relatively fast, allows the detection...

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Bibliographic Details
Published in:Food chemistry 2004-01, Vol.84 (3), p.485-491
Main Authors: Aristoy, M.-Concepción, Soler, Cristina, Toldrá, Fidel
Format: Article
Language:English
Subjects:
Animal proteins in feeds
Anserine
Balenine
Biological and medical sciences
Carnosine
Feed and pet food industries
Food industries
Fundamental and applied biological sciences. Psychology
General aspects
Histidine dipeptides
Methods of analysis, processing and quality control, regulation, standards
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Summary:A simple methodology, consisting in cation exchange HPLC separation combined with OPA postcolumn detection, has been succesfully applied to the analysis and detection of histidine dipeptides in feeds. The use of the proposed methodology, which is very simple and relatively fast, allows the detection of 2.63 ppm and 0.58 ppm of carnosine and anserine, respectively, in cattle feed. As balenine (3-methylhistidine) was not commercialy available, it was isolated from pork muscle and used as standard for its quantitation in feeds. These dipeptides have shown good stability against intense heat treatments, even at temperatures as high as 120 °C for 20 min. So, these histidine dipeptides can be feasible used as markers of animal protein content in feeds.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2003.07.030