Determination of vitamin K1 content in olive oil, chard and human plasma by RP-HPLC method with UV-Vis detection

The contents of phylloquinone (Vitamin K1) were investigated in olive oil, chard and human plasma based on a reversed phase high performance liquid chromatographic method with acetonitrile/dichloromethane/methanol (60:20:20, v/v/v) as eluent in a C18 μ-Bondapak column (10 micrometer, 300 x 3.9 mm) a...

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Bibliographic Details
Published in:Food chemistry 2007, Vol.100 (3), p.1220-1222
Main Authors: Otles, S, Cagindi, O
Format: Article
Language:English
Subjects:
assays
blood plasma
chard
detection
food analysis
green leafy vegetables
human nutrition
nutrient content
olive oil
phylloquinone
quantitative analysis
rapid methods
reversed-phase high performance liquid chromatography
ultraviolet radiation
vitamin K
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Summary:The contents of phylloquinone (Vitamin K1) were investigated in olive oil, chard and human plasma based on a reversed phase high performance liquid chromatographic method with acetonitrile/dichloromethane/methanol (60:20:20, v/v/v) as eluent in a C18 μ-Bondapak column (10 micrometer, 300 x 3.9 mm) at 20 °C. Detection was by UV-Vis detector at 248 nm. In olive oil, the mean content of phylloquinone ranged from 12.7 to 18.9 μg/100 g, in chard ranged from 65.5 to 77.5 μg/100 g. In human plasma, phylloquinone content varied between 0.22 and 0.56 ng/ml.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2005.12.003