Gas chromatographic–mass spectrometric analysis of galactosyl derivatives obtained by the action of two different β-galactosidases

Di- and trisaccharides obtained by transgalactosylation reaction of lactose using two different commercial enzyme preparations (Pectinex Ultra SP-L and Lactozym 3000 L HP G) have been characterised by gas chromatography–mass spectrometry as their trimethylsilyl oximes. Although mass fragments are co...

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Bibliographic Details
Published in:Food chemistry 2009-06, Vol.114 (3), p.1099-1105
Main Authors: Cardelle-Cobas, A., Martínez-Villaluenga, C., Sanz, M.L., Montilla, A.
Format: Article
Language:English
Subjects:
beta-galactosidase
enzymatic transgalactosylation
enzymatic treatment
Galactooligosaccharides
galactosyl derivatives
Galactosyl-glycerol
gas chromatography
GC–MS
glycerol
glycosidic linkages
hydrolysis
lactose
Lactozym 3000 L HP G
mass spectrometry
Pectinex Ultra SP-L
Trimethylsilyl-oximes
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Summary:Di- and trisaccharides obtained by transgalactosylation reaction of lactose using two different commercial enzyme preparations (Pectinex Ultra SP-L and Lactozym 3000 L HP G) have been characterised by gas chromatography–mass spectrometry as their trimethylsilyl oximes. Although mass fragments are common for most of the glycosidic linkages, their relative abundances can be used to determine carbohydrate identity. Moreover, formation of galactosyl- and digalactosyl-glycerols from the reaction mixture of Pectinex Ultra SP-L with lactose has been described by first time. Evolution of these compounds during transgalactosylation reactions and their influence on galactooligosaccharides (GOS) synthesis have been also evaluated.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2008.10.057