Free radical scavenging activity and comparative proteomic analysis of antioxidative protein against H₂O₂-induced oxidative stress in neuronal cells

Artemisia annua was enzymatically hydrolyzed by five proteases and seven carbohydrases. All enzymatic extracts scavenged DPPH, hydroxyl and alkyl radicals. Especially, the Protamex among the various proteases and Maltogenase among the various carbohydrases extracts exhibited the highest scavenging a...

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Bibliographic Details
Published in:Food chemistry 2009-11, Vol.117 (2), p.232-240
Main Authors: Kim, Eun-Kyung, Lee, Seung-Jae, Moon, Sang-Ho, Jeon, Byong-Tae, Ahn, Chang-Bum, Kim, Bokyung, Lim, Beong-Ou, Park, Pyo-Jam
Format: Article
Language:English
Subjects:
antioxidant activity
antioxidative proteins
Artemisia annua
Biological and medical sciences
enzymatic hydrolysis
Food industries
free radical scavengers
Fundamental and applied biological sciences. Psychology
hydrogen peroxide
neurons
oxidative stress
plant extracts
proteomics
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Summary:Artemisia annua was enzymatically hydrolyzed by five proteases and seven carbohydrases. All enzymatic extracts scavenged DPPH, hydroxyl and alkyl radicals. Especially, the Protamex among the various proteases and Maltogenase among the various carbohydrases extracts exhibited the highest scavenging activity on hydroxyl radical. The extracts of A. annua clearly reduced neuronal cell death from H₂O₂-induced damage. In addition, a proteomic analysis, two-dimensional electrophoresis (2-DE) and matrix assisted laser desorption ionisation-time of flight/time of flight (MALDI-TOF/TOF) was used to identify the proteins of the neuronal cells whose expressions were or were not altered by the treatment of the Maltogenase extracts which showed the highest hydroxyl radical scavenging activity among all enzymatic extracts for 24h. The protein characterisation revealed that translation elongation factor Tu (EF-Tu), Immunoglobulin E (IgE) and voltage-dependent anion channel 1 (VDAC-1) were involved in the cell survival effects against H₂O₂-induced apoptosis. These results suggest that EF-Tu, IgE and VDAC-1 have an important role in the reduction of neuronal apoptosis by oxidative stress, and the enzymatic extracts of A. annua shows potent antioxidative activities by regulating EF-Tu, IgE and VDAC-1.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2009.03.115