Survey of 55 Turkish Salvia taxa for their acetylcholinesterase inhibitory and antioxidant activities

• Published in: Food chemistry 2010-05, Vol.120 (1), p.34-43
• Main Authors: Şenol, F. Sezer, Orhan, Ilkay, Celep, Ferhat, Kahraman, Ahmet, Doğan, Musa, Yilmaz, Gülderen, Şener, Bilge
• Format: Article
• Language: English
• Subjects: Acetylcholinesterase; Alzheimer disease; Alzheimer’s disease; antioxidant activity; Biological and medical sciences; DPPH radical scavenging activity; enzyme inhibition; enzyme inhibitors; Food industries; free radical scavengers; Fundamental and applied biological sciences. Psychology; Iron-chelating effect; medicinal plants; phytochemicals; plant extracts; Salvia; Salvia fruticosa; Total flavonoid; Total phenol
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2009.09.066

In the current work, our target was to screen inhibitory potentials of 55 Turkish Salvia taxa, 28 of which are endemic, against acetylcholinesterase (AChE), which is a chief enzyme in pathogenesis of Alzheimer’s disease (AD). The dichloromethane, ethyl acetate, and methanol extracts prepared from 55 Salvia taxa were tested for their AChE inhibitory activity at 25, 50, and 100 μg/ml using an ELISA microplate reader. The extracts were also screened for their scavenging effect against DPPH radical and iron-chelating capacity. Total phenol and total flavonoid contents of Salvia fruticosa were determined. Among the 165 Salvia extracts screened, only the dichloromethane extract of S. fruticosa showed inhibition towards AChE at 100 μg/ml having 51.07% of inhibition, while only the dichloromethane and ethyl acetate extracts of Salvia cilicica had a notable iron-chelating capacity at 100 μg/ml having 54.71% of chelating capacity. Most of the extracts showed remarkable scavenging effect against DPPH radical.