Identification of phenolic constituents of Cytisus multiflorus

• Published in: Food chemistry 2012-03, Vol.131 (2), p.652-659
• Main Authors: Pereira, Olívia R., Silva, Artur M.S., Domingues, Maria R.M., Cardoso, Susana M.
• Format: Article
• Language: English
• Subjects: apigenin; Apigenin derivatives; Biological and medical sciences; biological assessment; Chrysin-7-O-β-d-glucopyranoside; Cytisus; Electrospray ionisation-mass spectrometry; Flavones; flowers; Food industries; Fundamental and applied biological sciences. Psychology; high performance liquid chromatography; isomers; Luteolin derivatives; mass spectrometry; medicinal plants; NMR; nuclear magnetic resonance spectroscopy; phenolic compounds
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2011.09.045

► Cytisus multiflorus is mostly rich in chrysin-7-O-β-d-glucopyranoside. ► It contains moderate amounts of uncommon phenolic compounds for Fabaceae. ► Unique compounds enclose derivatives of common flavonoids. The phenolic composition of the ethanolic extract obtained from the flowers of the medicinal plant Cytisus multiflorus has been elucidated by high performance liquid chromatography, electrospray mass spectrometry and nuclear magnetic resonance analysis. The extract was mainly composed of flavones, including the common chrysin, orientin, luteolin-5-O-glucoside, luteolin-7-O-glucoside, apigenin and apigenin-7-O-glucoside, which appeared as minor components. The major flavone in the extract was chrysin-7-O-β-d-glucopyranoside, and it also contained moderate amounts of a dihydroxyflavone isomer of chrysin, as well as of 2″-O-pentosyl-6-C-hexosyl-luteolin, 2″-O-pentosyl-8-C-hexosyl-luteolin and 6″-O-(3-hydroxy-3-methylglutaroyl)-2″-O-pentosyl-C-hexosyl-apigenin, which are not commonly found in the Fabaceae family. Other novel phenolic compounds found in the ethanolic extract of C. multiflorus comprised the flavones 2″-O-pentosyl-6-C-hexosyl-apigenin, 2″-O-pentosyl-8-C-hexosyl-apigenin and 6″-O-(3-hydroxy-3-methylglutaroyl)-2″-O-pentosyl-C-hexosyl-luteolin. The assessment of the biological activities of the main compounds of this extract are now keen, in order to determine their relevance in the beneficial properties of the plant.