Constructing liposomal nanovesicles of ginseng extract against hydrogen peroxide-induced oxidative damage to L929 cells

► Liposomal nanovesicle (LN) as the nanocarrier for ginseng extract (GE). ► Antioxidant ability of GE reduces as homogenisation pressure and time increase. ► GELNs with higher PEG mol% give higher ginsenoside encapsulation and reducing powder. ► GELN presents higher ΔΨm and lower apoptotic rate in H...

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Published in:Food chemistry 2012-05, Vol.132 (2), p.744-751
Main Authors: Tsai, Wen-Che, Li, Wei-Chu, Yin, Hsin-Yi, Yu, Ming-Chiang, Wen, Hsiao-Wei
Format: Article
Language:English
Subjects:
Antioxidant activity
Apoptosis
Biological and medical sciences
Food industries
Fundamental and applied biological sciences. Psychology
General pharmacology
Ginseng extract
Liposomal nanovesicle
Medical sciences
Mitochondrial membrane potential
Pharmacognosy. Homeopathy. Health food
Pharmacology. Drug treatments
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Summary:► Liposomal nanovesicle (LN) as the nanocarrier for ginseng extract (GE). ► Antioxidant ability of GE reduces as homogenisation pressure and time increase. ► GELNs with higher PEG mol% give higher ginsenoside encapsulation and reducing powder. ► GELN presents higher ΔΨm and lower apoptotic rate in H2O2 damaged cells than GE. ► Encapsulation inside LN enhances the intracellular antioxidative activity of GE. In this study, a liposomal nanovesicle was applied as a nanocarrier of ginseng extract (GE) to enhance the intracellular antioxidant activity of GE. The optimal condition of preparing GE liposomal nanovesicle (GELN) was prepared by tagging with 3.0mol% of polyethenyl glycol (PEG3.0) followed by homogenisation at 15,000psi for 5min to reduce its diameter to ∼150nm. PEG3.0-GELN encapsulated the most ginsenosides (234.1±13.9mg/g) with a higher reducing power than GELNs tagged with lower amounts of PEG (0–1.5mol%). After treatment with PEG3.0-GELN (20μg ginsenoside/ml), the % increase in mitochondrial membrane potential (ΔΨ) of H2O2-damaged cells rose 20.9% and the % decrease in apoptosis increased 12.4%, compared with those treated with GE. Therefore, this investigation proved that utilising liposomal nanovesicles to encapsulate GE can effectively suppress the depolarisation of ΔΨ and thus enhance the survival rate of H2O2-damaged cells.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2011.11.026