Cytotoxic and antioxidant constituents from Garcinia subelliptica

• Published in: Food chemistry 2012-11, Vol.135 (2), p.851-859
• Main Authors: Lin, Kai-Wei, Huang, A-Mei, Yang, Shyh-Chyun, Weng, Jing-Ru, Hour, Tzyh-Chyuan, Pu, Yeong-Shiau, Lin, Chun-Nan
• Format: Article
• Language: English
• Subjects: Antioxidant; Antioxidants - analysis; Antioxidants - isolation & purification; Antioxidants - pharmacology; Biological and medical sciences; Cell Death - drug effects; Cell Line, Tumor; Cytotoxins - analysis; Cytotoxins - isolation & purification; Cytotoxins - pharmacology; Cytotoxity; Food industries; Fundamental and applied biological sciences. Psychology; Garcinia - chemistry; Garcinia subelliptica; Humans; Phloroglucinol; Plant Extracts - analysis; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Seeds - chemistry; Triterpenoid
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2012.04.133

► Two new triterpenoids and a new phloroglucinol were isolated from the seed of Garcinia subelliptica. ► Known phloroglucinols could protect the cisplatin toxicity through reduction of ROS in NTUB1 and identified as antioxidant. ► These findings showed that these constituents may be used as antioxidant. Two new triterpenoids, garcinielliptones Q (1) and S (3), and a new phloroglucinol, garcinielliptone R (2), were isolated from the seed of Garcinia subelliptica. Their structures were established by analysis of their spectroscopic data. Phloroglucinol, garcinielliptone FC (4) from this plant exhibited a significant increase of antiproliferative effect, while 4 combined with cisplatin significantly caused decrease of cell inhibition induced by cisplatin in NTUB1. Exposure of NTUB1 cells to 4 cotreated with cisplatin for significantly decreased the amount of reactive oxygen species (ROS) than that of the total amount generated by 4 and cisplatin. These results suggested that 4 could protect the cisplatin toxicity through reduction of ROS in NTUB1. Phloroglucinols, garcinielliptones, A (5) and F (7), and garsubelline A (6), from this plant, revealed ABTS radical cation scavenging activity and 5 displayed an inhibitory effect on xanthine oxidase. These finding showed that 5–7 may be used as antioxidants.