A simple extraction method for the detection and quantification of polyoxin D, a nucleoside antibiotic, in butterbur using UPLC-MS/MS

• Published in: Food chemistry 2017-04, Vol.221, p.683-688
• Main Authors: Rahman, Md. Musfiqur, Abd El-Aty, A.M., Kim, Sung-Woo, Na, Tae-Woong, Shin, Ho-Chul, Hong, Su-Myeong, Shim, Jae-Han
• Format: Article
• Language: English
• Subjects: Butterbur; Chromatography, High Pressure Liquid - methods; Dissipation; Fungicide; Half-life; Petasites - chemistry; Polyoxin D; Pyrimidine Nucleosides - chemistry; Tandem Mass Spectrometry - methods; UPLC-MS/MS
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2016.11.119

[Display omitted] •A simple and effective method was developed for the detection and quantification of polyoxin D in butterbur using UPLC-MS/MS.•Due to the hydrophilic character of polyoxin D, the analyte was unrecovered following QuEChERS extraction.•QuEChERS d-SPE was also unable to remove interference in the UPLC-MS/MS results originating from chlorophyll and other pigments from green leafy vegetables.•Use of acidified water extractions and Oasis HLB cartridges successfully recovered the target analyte. An effective analytical method was developed for the detection and quantification of polyoxin D in butterbur using ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Samples were extracted using acidified deionised water and purified via solid-phase extraction (SPE) using an HLB cartridge. An external matrix-matched standard calibration curve was prepared, which provided an excellent linearity with a coefficient of determination (R2) ⩾0.999. Limits of detection (LOD) and quantification (LOQ) were 0.015 and 0.0.05μg/g, respectively. The developed method was validated in terms of recovery performance using two fortification levels in triplicate. The storage stabilities of the various field samples were also determined. Our method provided consistent recovery (86.26–87.37%) with a relative standard deviation (RSD) of