Dynamic changes of enzymes involved in sugar and organic acid level modification during blueberry fruit maturation

•Dynamic changes of enzymes in sugar and acid biosynthesis were investigated.•Association between enzymes and major sugars and organic acids was analyzed.•Divergence and different regulation of multi-isoforms suggested their specific functions. In blueberry, sugars and organic acids determine fruit...

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Bibliographic Details
Published in:Food chemistry 2020-03, Vol.309, p.125617, Article 125617
Main Authors: Li, Xiaobai, Li, Chunnan, Sun, Jian, Jackson, Aaron
Format: Article
Language:English
Subjects:
Blueberry Plants - metabolism
Blueberry Plants - physiology
Enzymes - metabolism
Enzyme’s phylogeny
Enzyme’s regulation
Fruit - metabolism
Fruit - physiology
Malates - metabolism
Organic acid
Phylogeny
Plant Proteins - metabolism
Ripening
Soluble sugar
Sugars - metabolism
Vaccinium corymbosum
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Summary:•Dynamic changes of enzymes in sugar and acid biosynthesis were investigated.•Association between enzymes and major sugars and organic acids was analyzed.•Divergence and different regulation of multi-isoforms suggested their specific functions. In blueberry, sugars and organic acids determine fruit organoleptic quality and drastically change during fruit maturation. This study examined enzymes involved in the metabolism of sugars and organic acids during the three maturation phases (green, pink and blue). During maturation, an increase in sugar (mainly fructose and glucose) was associated with up-regulation of VcSPP (CUFF.32787.1), VcSPS (CUFF.14989.1), and VcINV (gene.g3367.t1.1, CUFF.8077.1 and CUFF.47310.2). A decrease in citrate was associated with VcACLY (CUFF.27347.1 and CUFF.28772.1) in the acetyl-CoA pathway and with VcGAD (CUFF.15663.1 and CUFF.13757.1) and VcGLT (CUFF.6416.1) in the GABA shunt. A decrease in malate was associated with VcMDH (CUFF.30072.1, CUFF.18332.1 and CUFF.24878.1) involved in malate biosynthesis, and with VcADH (gene.g1507.t1.1, CUFF.3210.1 and gene.g30667.t1.1) as well as VcPDC (CUFF.47532.1) involved in fermentation. Multi-isoforms of enzymes were divergent and differentially regulated, suggesting that they have specialized functions in these pathways. The information will contribute to the understanding of blueberry organoleptic quality.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2019.125617