Bioaccessibility and cellular uptake by Caco-2 cells of carotenoids and chlorophylls from orange peels: A comparison between conventional and ionic liquid mediated extractions

• Published in: Food chemistry 2021-03, Vol.339, p.127818, Article 127818
• Main Authors: Murador, Daniella C., De Souza Mesquita, Leonardo M., Neves, Bruna V., Braga, Anna R.C., Martins, Paula L.G., Zepka, Leila Q., De Rosso, Veridiana V.
• Format: Article
• Language: English
• Subjects: [C4mim]Cl; Biological Availability; Caco-2 Cells; Carotenoids - analysis; Carotenoids - isolation & purification; Carotenoids - pharmacokinetics; Chemical Fractionation - methods; Chlorophyll - analysis; Chlorophyll - isolation & purification; Chlorophyll - pharmacokinetics; Chromatography, High Pressure Liquid; Citrus; Citrus sinensis - chemistry; Digestion; Esters; Esters - pharmacokinetics; Fruit - chemistry; Humans; Ionic Liquids - chemistry; Micellarization; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacokinetics; Tandem Mass Spectrometry; Xanthophyll; Xanthophylls - analysis; Xanthophylls - isolation & purification; Xanthophylls - pharmacokinetics
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2020.127818

•Native carotenoids extract obtained with ionic liquid was evaluated.•Bioaccessibility and cellular uptake of chlorophylls from orange peel were analyzed.•Bioaccessibility and cellular uptake of carotenoids esters were determined.•Xanthophylls and carotenes presented higher bioaccessibility than carotenoid esters.•Uptakes by Caco-2 cells of xanthophylls and esters were higher than of carotenes. Native extracts from orange peels were obtained by a conventional method using acetone and, an alternative method using ionic liquid (1-butyl-3-methylimidazolium chloride ([C4mim]Cl)). The bioaccessibilities and cellular uptakes of carotenoids, esters and chlorophylls were evaluated, since the influence of esterification on bioaccessibility and bioavailability is not well established. For this, the extracts were emulsified, submitted to in vitro simulated digestion model according to the INFOGEST protocol, followed by uptake by Caco-2 cells. Compounds were separated, identified and quantified by HPLC-PDA-MS/MS. After digestion, 22.0% and 26.2% of the total carotenoids and 45.9% and 68.7% of the chlorophylls were bioaccessible from the acetone and [C4mim]Cl extracts, respectively. The bioaccessibilities of xanthophylls and carotenes were significantly higher than those of the mono- and diesters. The uptake by Caco-2 cells varied from 130.2 to 131.9 ng/mg cell protein for total carotenoids and from 243.8 to 234.2 ng/mg cell protein for chlorophylls in the acetone and [C4mim]Cl extracts, respectively. In general, xanthophylls and esters were better absorbed than carotenes.