Visible on-site detection of Ara h 1 by the switchable-linker-mediated precipitation of gold nanoparticles

•Switchable linker (SL)-based assay could detect Ara h 1 in the field.•The SL was switched off by Ara h 1, which generated a visual signal.•Ara h 1 in 0.19 mg of peanut protein per 30 g of cookie was detectable.•Total assay time was less than 60 min, without the use of analytical equipment. Biosenso...

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Bibliographic Details
Published in:Food chemistry 2021-08, Vol.352, p.129354, Article 129354
Main Authors: Kim, Eunghee, Hahn, Jungwoo, Ban, Choongjin, Jo, Youngje, Han, Hyebin, Lim, Seokwon, Choi, Young Jin
Format: Article
Language:English
Subjects:
Antigens, Plant - analysis
Antigens, Plant - immunology
Antigens, Plant - isolation & purification
Ara h 1
Chemical Precipitation
Gold - chemistry
Humans
Membrane Proteins - analysis
Membrane Proteins - immunology
Membrane Proteins - isolation & purification
Metal Nanoparticles - chemistry
Peanut Hypersensitivity
Plant Proteins - analysis
Plant Proteins - immunology
Plant Proteins - isolation & purification
Precipitation of gold nanoparticles (AuNP)
Switchable linker (SL)
Visible on-site detection
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Summary:•Switchable linker (SL)-based assay could detect Ara h 1 in the field.•The SL was switched off by Ara h 1, which generated a visual signal.•Ara h 1 in 0.19 mg of peanut protein per 30 g of cookie was detectable.•Total assay time was less than 60 min, without the use of analytical equipment. Biosensors have been widely applied in tests for allergens, but on-site detection remains a challenge. Herein, we proposed a detection procedure for peanut Ara h 1 as a representative allergen, which was extracted from a cookie, thereby minimising the need for any complex pretreatment that was difficult to perform, and enabling the visual detection of the target without the use of analytical equipment. The extraction procedure was performed in less than 30 min using a syringe and filter (0.45 μm). The detection method for Ara h 1 was based on the aggregation of switchable linkers (SL) and gold nanoparticles (AuNP), and the presence of 0.19 mg peanut protein per 30 g of cookie could be confirmed within 30 min based on the AuNP/SL concentration ratio by the precipitation. This proposed procedure could be successfully applied to the detection of a wide range of food allergens.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2021.129354