Antioxidant capacity of 12 major soybean isoflavones and their bioavailability under simulated digestion and in human intestinal Caco-2 cells

[Display omitted] •Acetyl conjugate of isoflavones in the intestinal digestion had the lowest stability.•Isoflavone aglycones had the highest permeability and cellular accumulation.•Glycitein had the lowest permeability and cellular accumulation among 12 isoflavones.•Caco-2 cells can use acetyl and...

Full description

Saved in:
Bibliographic Details
Published in:Food chemistry 2022-04, Vol.374, p.131493, Article 131493
Main Authors: Kim, Mi-Seon, Jung, Young Sung, Jang, Davin, Cho, Chi Heung, Lee, Sang-Hoon, Han, Nam Soo, Kim, Dae-Ok
Format: Article
Language:English
Subjects:
Antioxidants
Bioaccessibility
Biological Availability
Caco-2 Cells
Cellular accumulation
Digestion
Digestion stability
Glycine max
Humans
Isoflavone conjugate
Isoflavones
Permeability
Transwell system
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:[Display omitted] •Acetyl conjugate of isoflavones in the intestinal digestion had the lowest stability.•Isoflavone aglycones had the highest permeability and cellular accumulation.•Glycitein had the lowest permeability and cellular accumulation among 12 isoflavones.•Caco-2 cells can use acetyl and malonyl conjugates of isoflavones in intact form. Soy isoflavones (SIs) show various health benefits, such as antioxidant and estrogenic effects. It is important to understand the bioaccessibility and bioavailability of SIs due to the close relation to their bioactivities. In this study, the antioxidant capacity, bioaccessibility, and bioavailability of 12 SIs were evaluated using radical-scavenging methods, simulations of human digestion, and Caco-2 cells in Transwell, respectively. All SIs were stable (91.1–99.2%) under gastric digestion conditions compared with the control (100%), whereas acetyl and malonyl conjugates were unstable (38.5% and 65.5%, respectively) under small intestinal digestion conditions. SI aglycones showed higher permeability (7–15 times) and cellular accumulation (8.8 times) than their glucosides. A small amount of SI conjugates was intact in the cell and in the basolateral side of each Transwell. These results suggest that SI conjugates, especially malonyl and acetyl forms, have incidental bioactivity after being metabolized to aglycones inside the cell.
ISSN:0308-8146
1873-7072
DOI:10.1016/j.foodchem.2021.131493