Development of anti-zearalenone monoclonal antibody and detection of zearalenone in corn products from China by ELISA

High-throughput screening enzyme-linked immunosorbent assay (HTS-ELISA) method was used for screening of hybridoma cells that secret high-affinity anti-zearalenone (ZON) monoclonal antibody (mAb). Recovery rate of ZON from spiked corn samples was between 80% and 128%. The cross-reactivities of the a...

Full description

Saved in:
Bibliographic Details
Published in:Food control 2013-05, Vol.31 (1), p.65-70
Main Authors: Pei, Shi-Chun, Lee, Won-Jong, Zhang, Gai-Ping, Hu, Xiao-Fei, Eremin, Sergei A., Zhang, Li-Jun
Format: Article
Language:English
Subjects:
aflatoxin B1
antibody detection
China
Corn
corn products
deoxynivalenol
detection limit
enzyme-linked immunosorbent assay
farms
HTS-ELISA
hybridomas
inhibitory concentration 50
monoclonal antibodies
screening
supermarkets
Zearalenone
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:High-throughput screening enzyme-linked immunosorbent assay (HTS-ELISA) method was used for screening of hybridoma cells that secret high-affinity anti-zearalenone (ZON) monoclonal antibody (mAb). Recovery rate of ZON from spiked corn samples was between 80% and 128%. The cross-reactivities of the anti-ZON mAb against ZON, β-zearalenol, α-zearalanol, β-zearalanol, deoxynivalenol and aflatoxin B1 were 100%, 24.1%, 189.1%, 43.9%,
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2012.09.006