Influence of different mobile phase compositions on detection of Ochratoxin A

Ochratoxin A (OTA) is a possible human carcinogen that can be found in a variety of foodstuffs. High performance liquid chromatography (HPLC) is the chosen method for the quantification of OTA in food for human and animal consumption. The aim of the present study was to investigate the influence of...

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Bibliographic Details
Published in:Food control 2013-05, Vol.31 (1), p.244-250
Main Authors: Afsah-Hejri, L., Jinap, S.
Format: Article
Language:English
Subjects:
acetic acid
Acid
Biological and medical sciences
Excitation wavelength
Food industries
Food toxicology
foods
Fundamental and applied biological sciences. Psychology
General aspects
high performance liquid chromatography
humans
Methods of analysis, processing and quality control, regulation, standards
Mobile phase composition
Ochratoxin A
Quantification value
Salt
wavelengths
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Summary:Ochratoxin A (OTA) is a possible human carcinogen that can be found in a variety of foodstuffs. High performance liquid chromatography (HPLC) is the chosen method for the quantification of OTA in food for human and animal consumption. The aim of the present study was to investigate the influence of different mobile phase compositions on the quantification (as measured by the peak area) of OTA. Different mobile phases were designed to study the effects of the mobile phase composition, pH, type and molarity of salt, the addition of acid and the chosen excitation wavelength on the detection of OTA. Results from thirteen mobile phase compositions showed that the mobile phase composition of ACN/NaAcetate (5 mM)/MeOH/Acetic acid (pH 3); (30:40:30; v/v/v) resulted in the highest quantification value for OTA. The designed mobile phase provided a significantly (p 
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2012.09.027