Carbohydrate-coated nanoparticles for PCR-less genomic detection of Salmonella from fresh produce

Foodborne outbreaks related to Salmonella are a growing concern as it is commonly associated with raw and uncooked food. Traditional methods for extracting and detecting this pathogen from complex food matrices can be time-consuming and expensive. This study used carbohydrate-coated magnetic and gol...

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Bibliographic Details
Published in:Food control 2023-08, Vol.150, p.109770, Article 109770
Main Authors: Sharief, Saad Asadullah, Caliskan-Aydogan, Oznur, Alocilja, Evangelyn C.
Format: Article
Language:English
Subjects:
Biosensor
Food safety
Foodborne pathogens
Salmonella
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Summary:Foodborne outbreaks related to Salmonella are a growing concern as it is commonly associated with raw and uncooked food. Traditional methods for extracting and detecting this pathogen from complex food matrices can be time-consuming and expensive. This study used carbohydrate-coated magnetic and gold nanoparticles to successfully extract and detect Salmonella Enteritidis from artificially contaminated cucumbers and melons. Rapid separation of bacterial cells from buffer solution and food matrices using glycan-coated magnetic nanoparticles (gMNP) was confirmed using Transmission Electron Microscopy (TEM). Quantitative PCR verified the successful magnetic extraction of S. Enteritidis from artificially contaminated foods. Genomic detection was achieved using dextrin-capped gold nanoparticles (dGNP) synthesized using alkaline synthesis route. The synthesized dGNP were wine-red in color, and dynamic light scattering confirmed their size to be 
ISSN:0956-7135
1873-7129
DOI:10.1016/j.foodcont.2023.109770