Rapid detection of the freshness of grass carp (Ctenopharygodon idella) fillets by colloidal gold immunochromatography assay

• Published in: Food control 2024-05, Vol.159, p.110303, Article 110303
• Main Authors: Teng, Jialu, Chen, Hong, Yang, Fang, Yu, Dawei, Gao, Pei, Yu, Peipei, Jiang, Qixing, Xu, Yanshun, Xia, Wenshui, Yu, Dongxing
• Format: Article
• Language: English
• Subjects: Colloidal gold immunochromatographic assay; Freshness; Freshwater fish fillets; Parvalbumin; Protein indicator
• ISSN: 0956-7135; 1873-7129
• DOI: 10.1016/j.foodcont.2024.110303

Consumers are greatly concerned about fish freshness. Therefore, this study was aimed to provide a colloidal gold immunochromatography assay (GICA) system for rapid detection of fish freshness. A suitable fish protein was chosen as an antigen for GICA; and the properties and effectiveness of the GICA system were investigated. Results showed that parvalbumin (PV) was suitable as a fish freshness indicator, proven by hierarchical cluster analysis and chosen as a GICA antigen. The GICA system revealed high specificity in the 27–212 μg/L detection range. The correlation coefficient R2 towards enzyme-linked immunosorbent assay reflected high reliability as 0.998. The GICA system was stable within 12 weeks at room temperature. The fitness for indicating freshness by GICA ranged from 87.27% to 111.72%. In short, the GICA system with PV as an antigen could be used as a simple, rapid, and accurate technique for the freshness detection of grass carp fillets. •Fish freshness was classified into three levels by hierarchical cluster analysis.•Parvalbumin (PV) in sarcoplasm and drip could indicate grass carp freshness.•PV from grass carp was purified effectively.•Colloidal gold immunochromatographic assay for fish freshness was established and effective.•The detection in drip sample was better than sarcoplasmic protein sample.