Norbixin binding to whey protein isolate - alginate electrostatic complexes increases its solubility and stability

Norbixin is a carotenoid extracted from the Annatto plant (Bixa orellana) and widely used as a natural colorant in pharmaceuticals, cosmetics and food. It is water-soluble at neutral and alkaline pH, but below neutral pH, norbixin starts to precipitate limiting the use in acidic food products. As a...

Full description

Saved in:
Bibliographic Details
Published in:Food hydrocolloids 2020-04, Vol.101, p.105559, Article 105559
Main Authors: Møller, Anders Hauer, Wijaya, Wahyu, Jahangiri, Amita, Madsen, Bjoern, Joernsgaard, Bjarne, Vaerbak, Signe, Hammershøj, Marianne, Van der Meeren, Paul, Dalsgaard, Trine Kastrup
Format: Article
Language:English
Subjects:
Alginate
Colorant
Complexation
Encapsulation
Norbixin
Whey protein isolate
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Norbixin is a carotenoid extracted from the Annatto plant (Bixa orellana) and widely used as a natural colorant in pharmaceuticals, cosmetics and food. It is water-soluble at neutral and alkaline pH, but below neutral pH, norbixin starts to precipitate limiting the use in acidic food products. As a new approach to enhance the solubility and stability of norbixin in aqueous solution under acidic conditions, the colorant was applied in the presence of whey protein isolate (WPI) and alginate (ALG). Addition of WPI prevented norbixin precipitation between pH 2 and pH 7 except at the pI of WPI, pH 5. The visual appearance, colorimetric measurements and a blue shift in the absorbance spectrum indicated that norbixin formed H-aggregates at pH 
ISSN:0268-005X
1873-7137
DOI:10.1016/j.foodhyd.2019.105559