Obtaining glycosaminoglycans from tilapia (oreochromis niloticus) scales and evaluation of its anticoagulant and cytotoxic activities

[Display omitted] •The presence of GAGs in tilapia scales and their bioactivities was studied.•Chondroitin sulfate chains CS-A and CS-C were found.•The chondroitin sulfates showed anticoagulant activity.•Chondroitin sulfates showed a cytotoxic effect for metabolizing tumor cells.•Tilapia scales can...

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Bibliographic Details
Published in:Food research international 2021-02, Vol.140, p.110012, Article 110012
Main Authors: de Moura, Heloisa C., Novello, Claudio R., Balbinot-Alfaro, Evellin, Düsman, Elisângela, Barddal, Helyn P.O., Almeida, Igor V., Vicentini, Veronica E.P., Prentice-Hernández, Carlos, Alfaro, Alexandre T.
Format: Article
Language:English
Subjects:
Bioactivities
By-products
Chondroitin sulfate
CS-A
CS-C
GAGs
Underutilized fish parts
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Summary:[Display omitted] •The presence of GAGs in tilapia scales and their bioactivities was studied.•Chondroitin sulfate chains CS-A and CS-C were found.•The chondroitin sulfates showed anticoagulant activity.•Chondroitin sulfates showed a cytotoxic effect for metabolizing tumor cells.•Tilapia scales can be an alternative source to the extraction of chondroitin sulfate. Large amounts of by-products are generated during fish processing. The study aimed to assess whether tilapia scales are a potential source for obtaining glycosaminoglycans, as well as to determine their anticoagulant and cytotoxic/antiproliferative activities, against different tumor lines. The glycosaminoglycans were extracted, purified, and fractionated. The fractions that indicated the presence of uronic acid and sulfated GAGs were characterized by electrophoresis, NMR, and degree of sulfation (DS). The extraction process using the papain enzyme had a yield of 0.86%. Fraction V (FV) revealed the presence of chondroitin sulfate chains CS-A and CS-C, with DS of 0.146. FV demonstrated anticoagulant potential, as it was able to increase aPTT time. FV showed a cytotoxic effect for HTC metabolizing cells at 24, 48, and 72 h. However, it did not show activity for neuroblastoma cells in any of the evaluated times. The results indicate that the tilapia scales are a possible source for obtaining chondroitin sulfate, with potential use as anticoagulant and cytotoxic/antitumor.
ISSN:0963-9969
1873-7145
DOI:10.1016/j.foodres.2020.110012