β-Amyloid-induced apoptosis is associated with cyclooxygenase-2 up-regulation via the mitogen-activated protein kinase–NF-κB signaling pathway

• Published in: Free radical biology & medicine 2005-06, Vol.38 (12), p.1604-1613
• Main Authors: Jang, Jung-Hee, Surh, Young-Joon
• Format: Article
• Language: English
• Subjects: COX-2; ERK; Inflammatory cell death; NF-κB; Oxidative stress; p38 MAPK; PC12 cells; β-Amyloid
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2005.02.023

Inflammatory cell death as well as oxidative stress has been implicated in some neurodegenerative disorders such as Alzheimer's disease (AD). Expression of cyclooxygenase-2 (COX-2) and production of prostaglandins have been frequently elevated in AD. In this study, we have investigated the molecular mechanisms underlying inflammatory cell death induced by β-amyloid (Aβ), a neurotoxic peptide that accumulates in senile plaques formed in the brains of AD patients. Rat pheochromocytoma (PC12) cells treated with Aβ exhibited increased mRNA and protein expression of COX-2 and production of prostaglandin E 2 (PGE 2) and underwent apoptotic death as determined by positive in situ terminal end-labeling, decreased mitochondrial membrane potential, increased Bax/Bcl-X L ratio, activation of c-Jun N-terminal kinase, and cleavage of poly(ADP-ribose)polymerase. Pretreatment with celecoxib, a selective COX-2 inhibitor, attenuated Aβ-induced cell death, which was aggravated by addition of the COX-2 product PGE 2. Aβ transiently induced activation of redox-sensitive transcription factor NF-κB, and pretreatment of PC12 cells with NF-κB inhibitors abolished the Aβ-induced COX-2 expression. Pharmacologic inhibition of extracellular signal-regulated kinase (ERK) and p38 mitogen-activated protein kinase (p38 MAPK) and dominant-negative mutation of both enzymes suppressed not only Aβ-induced NF-κB transactivation but also COX-2 expression and PGE 2 production. The above findings suggest that Aβ-induced apoptosis in PC12 cells is associated with COX-2 up-regulation through activation of NF-κB, which is mediated by upstream kinases including ERK and p38 MAPK.