Influence of oxidized low-density lipoproteins (LDL) on the viability of osteoblastic cells

Cardiovascular diseases have recently been noted as potential risk factors for osteoporosis development. Although it is poorly understood how these two pathologies are related, it is a known fact that oxidized low-density lipoproteins (OxLDL) constitute potential determinants for both of them. The c...

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Bibliographic Details
Published in:Free radical biology & medicine 2008-02, Vol.44 (4), p.506-517
Main Authors: Brodeur, Mathieu R., Brissette, Louise, Falstrault, Louise, Ouellet, Pascale, Moreau, Robert
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Cell death
Cell Line
Cell Survival - drug effects
Cholesteryl ester
Humans
Lipoproteins, LDL - metabolism
Lipoproteins, LDL - pharmacology
Lipoproteins, LDL - toxicity
Low-density lipoprotein
Lysosomes - drug effects
Mice
Osteoblast
Osteoblasts - drug effects
Osteoblasts - metabolism
Osteoporosis - etiology
Oxidation-Reduction
OxLDL
Proliferation
Selective uptake
Tetrazolium Salts - metabolism
Thiazoles - metabolism
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Summary:Cardiovascular diseases have recently been noted as potential risk factors for osteoporosis development. Although it is poorly understood how these two pathologies are related, it is a known fact that oxidized low-density lipoproteins (OxLDL) constitute potential determinants for both of them. The current study investigated the metabolism of OxLDL by osteoblasts and its effect on osteoblastic viability. The results obtained show that OxLDL are internalized but not degraded by osteoblasts while they can selectively transfer their CE to these cells. It is also demonstrated that OxLDL induce proliferation at low concentrations but cell death at high concentrations. This reduction of osteoblast viability was associated with lysosomal membrane damage caused by OxLDL as demonstrated by acridine orange relocalization. Accordingly, chloroquine, an inhibitor of lysosomal activity, accentuated cell death induced by OxLDL. Finally, we demonstrate that osteoblasts have the capacity to oxidize LDL and thereby potentially increase the local concentration of OxLDL. Overall, the current study confirms the potential role of OxLDL in the development of osteoporosis given its influence on osteoblastic viability.
ISSN:0891-5849
1873-4596
DOI:10.1016/j.freeradbiomed.2007.08.030