mRNA profiling for body fluid identification

mRNA profiling is a promising new method for the identification of body fluids from biological stains. A multiplex reverse transcription endpoint PCR method was adapted for the identification of blood, saliva, semen, vaginal secretions and menstrual blood. For specificity, sensitivity and suitabilit...

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Bibliographic Details
Published in:Forensic science international. Genetics supplement series 2008-08, Vol.1 (1), p.37-38
Main Authors: Haas, C., Klesser, B., Kratzer, A., Bär, W.
Format: Article
Language:English
Subjects:
Body fluid identification
mRNA profiling
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Summary:mRNA profiling is a promising new method for the identification of body fluids from biological stains. A multiplex reverse transcription endpoint PCR method was adapted for the identification of blood, saliva, semen, vaginal secretions and menstrual blood. For specificity, sensitivity and suitability to biological stains the endpoint PCR method was satisfying. Up to 15 months old stains were shown to be suitable for mRNA profiling. In addition a realtime PCR assay was tested for sensitivity with blood and semen samples, providing comparable results to endpoint PCR or enzymatic tests.
ISSN:1875-1768
1875-175X
DOI:10.1016/j.fsigss.2007.10.064