A dedicated automated system for extraction, quantification and STR amplification of forensic evidence samples

• Published in: Forensic science international. Genetics supplement series 2009-12, Vol.2 (1), p.64-65
• Main Authors: Stray, James, Nguyen, Vivian T., Benfield, Jacquelyn, Fang, Rixun, Brevnov, Maxim, Treat-Clemons, Lynda, Porter, Greg, Furtado, Manohar R., Shewale, Jaiprakash G.
• Format: Article
• Language: English
• Subjects: DNA analysis; DNA extraction; DNA genotyping; DNA quantification; Human identification; Real-time PCR; STR analysis
• ISSN: 1875-1768; 1875-175X
• DOI: 10.1016/j.fsigss.2009.08.083

Forensic DNA analysis is a multi-step process involving extraction of DNA, quantification of human DNA in the extract, amplification using multiplex STR systems, separation of products, and data analysis. The backlog of forensic casework is increasing worldwide. Automation is one significant way to alleviate the bottleneck of sample processing in forensic labs. The HID EVOlution™ Combination System described here is a robust, reliable sample processing platform, easily adapted to forensic laboratory workflows. Using a variety of forensic sample types including: blood stained FTA paper, cotton fabric and denim, dried blood spiked with known PCR inhibitors, saliva on cotton swabs, and semen stains, we found that yields of human DNA and STR profiles obtained with AmpF lSTR ® Idenitfiler ® kits were complete, highly reproducible, and equivalent to results obtained using the manual PrepFiler™ reagent extraction method. Automated operation was clean, and no cross-contamination was detected between extraction blanks and interspersed high DNA content samples.