High quality DNA from human remains obtained by using the Maxwell® 16 automated methodology

During the last two decades, a growing number of techniques has been experienced to maximize the quality and to reduce the risk of contamination of the DNA extracted from human remains and automated methodologies have been developed and introduced in some forensic laboratories. This work aimed to st...

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Bibliographic Details
Published in:Forensic science international. Genetics supplement series 2013, Vol.4 (1), p.e248-e249
Main Authors: Silva, D.A., Cavalcanti, P., Freitas, H., de Carvalho, E.F.
Format: Article
Language:English
Subjects:
Human remains
Insertion–deletion
Maxwell® 16
STR
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Summary:During the last two decades, a growing number of techniques has been experienced to maximize the quality and to reduce the risk of contamination of the DNA extracted from human remains and automated methodologies have been developed and introduced in some forensic laboratories. This work aimed to standardize conditions for extraction of DNA from human remains (bone) by using the Maxwell® 16 LEV system (Promega Corporation) and the DNA IQ™ Casework Sample Pro Kit for Maxwell® 16. Aiming to enlarge the amount of DNA having quality characteristics suitable for PCR amplification of SNP, INDELS and STR loci with different multiplexes systems a slight modification on the Maxwell® 16 original protocol has been included and demonstrated in this work. Considering STR genotyping results of bone human samples, it was found that the quality of the DNA produced by the Maxwell® 16 was higher than that of the DNA molecules obtained by the phenol/chloroform extraction procedure.
ISSN:1875-1768
1875-175X
DOI:10.1016/j.fsigss.2013.10.127