DNA extraction method from teeth using QIAcube

• Published in: Forensic science international. Genetics supplement series 2013, Vol.4 (1), p.e276-e277
• Main Authors: Pinchi, V., Focardi, M., Martinelli, D., Norelli, G.A., Carboni, I., Gozzini, A., Romolini, C., Torricelli, F., Ricci, U.
• Format: Article
• Language: English
• Subjects: DNA extraction; Forensic genetics; QIAcube; Teeth
• ISSN: 1875-1768; 1875-175X
• DOI: 10.1016/j.fsigss.2013.10.141

The use of semi-automated extraction from forensic samples is useful both for contamination control and method reproducibility. However, when an accredited ISO17025 forensic laboratory starts to use a new analytical system, an internal validation procedure is needed before processing forensic samples. Here we show validation results obtained by introducing QIAcube automated station (Qiagen) for DNA extraction from teeth that enables seamless integration of automated low-throughput sample preparation into the laboratory workflow. Purification steps, in association with QIAamp DNA Investigator Kit, are thus fully automated, in order to prevent cross contamination. A total of 18 teeth stored for 4–5 years after removal and 8 teeth stored for one-two months at room temperature underwent to DNA extraction by using this system. Teeth were previously decontaminated and a conservative endodontic access to pulp chamber was performed. A blood reference of the teeth donors was also collected. After DNA extraction all samples were quantified by using Quantifiler® Duo DNA Quantification Kit. Total DNA ranging between 0.2 and 23,900ng in 50μl of TE buffer with no inhibitors presence in samples. The obtained results confirm the robustness and reproducibility of the extraction method with QIAcube technology.