Testing the behavior of GlobalFiler® PCR amplification kit with degraded and/or inhibited biological samples

The achievement of complete genetic profiles of degraded and/or inhibited biological samples is a challenging task for forensic scientists. Our aim was to find out the behavior of GlobalFiler® PCR amplification kit in the presence of difficult samples and its ability to substitute or to be complemen...

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Bibliographic Details
Published in:Forensic science international. Genetics supplement series 2015-12, Vol.5, p.e21-e23
Main Authors: Bogas, V., Carvalho, M., Corte-Real, F., Porto, M.J.
Format: Article
Language:English
Subjects:
AmpFℓSTR® MiniFiler
Degraded DNA
GlobalFiler
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Summary:The achievement of complete genetic profiles of degraded and/or inhibited biological samples is a challenging task for forensic scientists. Our aim was to find out the behavior of GlobalFiler® PCR amplification kit in the presence of difficult samples and its ability to substitute or to be complemented by AmpFℓSTR® MiniFiler™ kit. Degraded bloodstains were extracted by three methods, quantified and amplified with AmpFℓSTR®IdentiFiler™, AmpFℓSTR® MiniFiler™ and/or GlobalFiler® PCR. In 6 of the 14 analyzed samples, GlobalFiler® kit enabled a complete genetic profile even when the sample had a DNA concentration as low as 0.0049ng/ml, while in some of these samples Minifiler™ provided a partial profile or didn't amplify any of the markers in study.
ISSN:1875-1768
1875-175X
DOI:10.1016/j.fsigss.2015.09.009