Optimization of the collection and analysis of touch DNA traces

The aim of this work was to optimize a strategy for detection, collection, and analysis of touch DNA traces. 4N6FLOWSwabs™ and cotton swabs were compared by collecting touch DNA traces from glass slides and gun shell casings. Shed cells were visualized using Diamond™ nucleic acid dye and a digital f...

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Bibliographic Details
Published in:Forensic science international. Genetics supplement series 2019-12, Vol.7 (1), p.98-99
Main Authors: Haase, H.T., Mogensen, H.S., Petersen, C.B., Petersen, J.F., Holmer, A., Børsting, C., Pereira, V.
Format: Article
Language:English
Subjects:
DNA collection
Low template DNA
Trace samples
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Summary:The aim of this work was to optimize a strategy for detection, collection, and analysis of touch DNA traces. 4N6FLOWSwabs™ and cotton swabs were compared by collecting touch DNA traces from glass slides and gun shell casings. Shed cells were visualized using Diamond™ nucleic acid dye and a digital fluorescent microscope. Different collection and extraction methods were tested. Collected samples were amplified with the AmpFlSTR® NGMSElect™ kit and the Precision ID mtDNA Whole Genome Panel.
ISSN:1875-1768
1875-175X
DOI:10.1016/j.fsigss.2019.09.038