Implementation of Prep-n-Go™ Buffer for DNA extraction from buccal swabs

The efficacy of two extraction methods; room temperature and heat protocols was assessed for buccal swabs using the Prep-n-Go™ Buffer. DNA was extracted from buccal swabs using both extraction methods and their effectiveness to produce good quality DNA profiles was evaluated. Heat protocol was found...

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Bibliographic Details
Published in:Forensic science international. Genetics supplement series 2019-12, Vol.7 (1), p.211-213
Main Authors: Iyavoo, S., Knights, S., Mavrommatis, M., Haizel, T.
Format: Article
Language:English
Subjects:
Buccal swab
DNA extraction
Prep-n-Go™ Buffer
VeriFiler™ Express
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Summary:The efficacy of two extraction methods; room temperature and heat protocols was assessed for buccal swabs using the Prep-n-Go™ Buffer. DNA was extracted from buccal swabs using both extraction methods and their effectiveness to produce good quality DNA profiles was evaluated. Heat protocol was found to yield more DNA, however room temperature protocol produced better quality DNA profiles with fewer artefacts when the samples from both extraction methods were amplified directly without any normalisation with the VeriFiler™ Express PCR Amplification Kit.
ISSN:1875-1768
1875-175X
DOI:10.1016/j.fsigss.2019.09.081