A novel prognostic biomarker for pancreatic ductal adenocarcinoma: hsa_circ_0001649

• Published in: Gene 2018-10, Vol.675, p.88-93
• Main Authors: Jiang, Yuehong, Wang, Tong, Yan, Li, Qu, Lijuan
• Format: Article
• Language: English
• Subjects: Adult; Aged; Aged, 80 and over; Biomarker; Biomarkers, Tumor - genetics; Carcinogenesis - genetics; Carcinoma, Pancreatic Ductal - diagnosis; Carcinoma, Pancreatic Ductal - genetics; Carcinoma, Pancreatic Ductal - pathology; Cell Line, Tumor; Cell Proliferation - genetics; Circular RNA; Female; Gene Expression Regulation, Neoplastic; hsa_circ_0001649; Humans; Male; Middle Aged; Pancreatic ductal adenocarcinoma; Pancreatic Neoplasms - diagnosis; Pancreatic Neoplasms - genetics; Pancreatic Neoplasms - pathology; RNA - genetics
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/j.gene.2018.06.099

Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease with increasing incidence worldwide. Accumulating evidence indicated that circular RNAs (circRNAs) behave as a novel class of transcription products during multiple cancer processes. Specifically, hsa_circ_0001649 has been reported to be down-regulated in several cancers. However, its clinical significance and functional roles in PDAC is still unknown. RT-qPCR was carried out to measure the expression of hsa_circ_0001649 in PDAC tissue samples and cell lines. Additionally, the correlation between hsa_circ_0001649 expression and clinicopathological features was analyzed. The prognostic role of hsa_circ_0001649 was explored by Cox regression analysis. The potential effects of hsa_circ_0001649 in PDAC cells were evaluated in vitro including cell proliferation, colony-forming ability and apoptosis. As a result, hsa_circ_0001649 was abnormally decreased in PDAC tissues and cells, and this down-regulation was correlated with tumor stage and differentiation grade in PDAC patients. Hsa_circ_0001649 could serve as an independent prognostic factor for PDAC patients after surgery. What's more, increased hsa_circ_0001649 caused tumor suppressive effects via reducing cell proliferation, colony-forming ability and promoting cell apoptosis in PANC1 and BxPC3 cells. Collectively, the results illustrated that hsa_circ_0001649 may play a tumor suppressor role in PDAC and offer a potential therapeutic target for treating this fatal disease. •Hsa_circ_0001649 is downregulated in pancreatic ductal adenocarcinoma both in tissues and cells.•Overexpression of hsa_circ_0001649 inhibits cell growth and promotes cell apoptosis in PDAC cells.•Low expression of hsa_circ_0001649 is associated with advanced tumor stage and low differentiation grade.•Patients with low expression of hsa_circ_0001649 had poorer overall survival.