ARID3A-mediated modulation of TP73 and TP73-AS1 in osteosarcoma cells

Osteosarcoma is the most common primary malignancy arising from bone. Increasing mass of indications suggest that long non-coding RNAs (lncRNAs) play crucial roles in the development of progressions of human cancers including osteosarcoma. Although several lncRNAs have shown to be involved in the mo...

Full description

Saved in:
Bibliographic Details
Published in:Gene reports 2020-09, Vol.20, p.100683, Article 100683
Main Authors: Saadat, Khandakar A.S.M., Bozgeyik, Esra, Arman, Kaifee, Bozgeyik, Ibrahim, Ikeda, Masa-Aki
Format: Article
Language:English
Subjects:
ARID3A
lncRNA
non-coding RNA
Osteosarcoma
TP73
TP73-AS1
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Osteosarcoma is the most common primary malignancy arising from bone. Increasing mass of indications suggest that long non-coding RNAs (lncRNAs) play crucial roles in the development of progressions of human cancers including osteosarcoma. Although several lncRNAs have shown to be involved in the molecular pathogenesis of osteosarcoma, identification of novel lncRNAs involved in the osteosarcoma pathobiology remained muchly elusive. Besides, ARID3A is a member of ARID family of DNA binding proteins. ARID3A was also implicated in osteosarcoma pathogenesis. Accordingly, in the present study, we mechanistically investigated the effect of ARID3A on the expression of TP73 and TP73-AS1 in osteosarcoma cells and to determine the relationship between them. For the overexpression of ARID3A in U2-OS cells, pER_xpress_ARID3A expression vector and for the silencing of ARID3A, ARID3A-spesific siRNA was used. Expression levels of ARID3A, TP73 and TP73-AS1 genes were determined by qPCR method. As a result, expression levels of TP73-AS1 were well-correlated with the ARID3A expression levels whereas TP73 expression levels were not well-correlated with ARID3A. In conclusion, our results indicate that ARID3A might be involved in the regulation of TP73-AS1 in osteosarcoma. To our knowledge, this is the first study revealing the role of ARID3A in the regulation of TP73 and TP73-AS1 genes. •Osteosarcoma is the most common primary malignancy arising from bone.•ARID3A is a member of ARID family of DNA binding proteins.•ARID3A might be involved in the regulation of TP73-AS1 in osteosarcoma.•TP73-AS1 was upregulated in U-2 OS cells overexpressing ARID3A.•TP73-AS1 was downregulated in ARID3A-silenced U-2 OS cells.ss
ISSN:2452-0144
2452-0144
DOI:10.1016/j.genrep.2020.100683