Serum and tissue expression levels of microRNAs-661, -571 and -770-5p among diabetic foot ulcer patients compared to healthy controls

Micro-RNAs (miRNAs) are considered as biomarkers playing a role for prediction of type 2 diabetes mellitus (T2DM) and diabetic foot ulcer (DFU). More relevantly, miRNAs confer higher sensitivity levels than currently used tools. In this study, we aimed to evaluate serum and tissue expression levels...

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Bibliographic Details
Published in:Gene reports 2020-12, Vol.21, p.100946, Article 100946
Main Authors: Alhatemi, Abdulkareem Khattar, Majeed, Suhad Rasheed, Mubarak, Shaden M.H.
Format: Article
Language:English
Subjects:
Biomarkers
Diabetic foot ulcer
MicroRNAs
Type-2 diabetes mellitus
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Summary:Micro-RNAs (miRNAs) are considered as biomarkers playing a role for prediction of type 2 diabetes mellitus (T2DM) and diabetic foot ulcer (DFU). More relevantly, miRNAs confer higher sensitivity levels than currently used tools. In this study, we aimed to evaluate serum and tissue expression levels of miR-770-5p, miR-571 and miR-661 among T2DM and DFU patients compared to healthy control population. The study included 600 subjects (300 patients, 150 healthy control individuals and 150 individuals with trauma injury). Sampling was taken from T2DM (blood) and DFU patients (blood and tissue), healthy subjects (blood control samples) and individuals with trauma injury (tissue control samples). Serum and tissue expression levels of miR-770-5p, miR-571 and miR-661 were measured using quantitative Real time PCR (RT-qPCR) technique. The miR-661 expression was significantly higher in tissue and sera samples of patients with DFU compared to control groups (p 
ISSN:2452-0144
2452-0144
DOI:10.1016/j.genrep.2020.100946