The negative correlation between miR-140-3-p and Metadherin gene in estrogen and progesterone receptor positive–breast cancer

• Published in: Gene reports 2021-06, Vol.23, p.101068, Article 101068
• Main Authors: Nabil, Mai Mohamed, Ahmed, Maha Imam, Al-Sheeha, Muneera, Yousry, Walaa Ahmed, Mohamed, Dalia Abdel Wahab
• Format: Article
• Language: English
• Subjects: Biomarker; Breast cancer; Estrogen receptors; Metadherin; Mir-140-3p; Progesterone receptors
• ISSN: 2452-0144; 2452-0144
• DOI: 10.1016/j.genrep.2021.101068

Breast cancer is a complicated cancer with different etiologies including both environmental and genetic factors. Aberrant expression of many genes and proteins is essential for its development and progression. Metadherin (MTDH) gene is one of the tumor related genes that involved in tumorigenesis and metastasis. MicroRNAs (miRNAs) are widely involved in cell proliferation, tumor invasion and apoptotic processes. Dysregulation of miRNAs expression was related to carcinogenesis and tumor progression. This study aimed to assess miR-140-3p expression and its possible target gene MTDH gene expression in breast tumor tissues. Moreover, we aimed to explore the correlation among their expression and different clinicopathological parameters. Bioinformatics analysis was done and revealed that MTDH is a one of many target genes for miR-140-3p. Reverse Transcription-quantitative Real-Time PCR (RT-qPCR) was used to identify the expression level of miR-140-3p and MTDH in 50 breast cancer tissues and 25 normal breast tissues. Results indicated that miR-140-3p was significantly lower in breast cancer tissues than normal breast tissues (P