Association between MEG3 polymorphisms (rs941576 and rs7158663) and risk of acute ischemic stroke in Egyptian patients

• Published in: Gene reports 2021-09, Vol.24, p.101286, Article 101286
• Main Authors: Shaker, Olfat, Sroor, Wafaa, Ali, Ola, Soliman, Hanan, Abdeen, Marwa
• Format: Article
• Language: English
• Subjects: Ischemic stroke; MEG3; rs7158663; rs941576
• ISSN: 2452-0144; 2452-0144
• DOI: 10.1016/j.genrep.2021.101286

Acute ischemic stroke (AIS) is a chief cause of mortality and debility worldwide. Maternally expressed gene 3 (MEG3), is highly expressed in brain and was found to be upregulated after AIS. Single nucleotide polymorphisms (SNPs) in MEG3 have been associated with various diseases. The current study aimed to detect whether MEG3 SNPs, rs941576 and rs7158663, are associated with AIS risk in Egyptians. samples were withdrawn from Egyptian subjects, 177 AIS patients and 73 controls, and genomic DNA was extracted to perform genotyping for rs941576 and rs7158663 using TaqMan Assays. A significantly higher risk of AIS was associated with rs941576 GG genotype before and after adjusting to age, gender, hypertension, and diabetes mellitus in the co-dominant (GG vs. AA: adjusted OR = 18.94, 95% CI: 2.38–50.52, P = 0.021) and dominant models (GG vs. AG + AA: adjusted OR = 18.095, 95% CI: 2.32–41.00, P = 0.006) and with G allele (G vs. A: adjusted OR = 2.20, 95% CI: 1.31–3.64, P = 0.003). Combined genotypes rs941576/rs7158663: AA/GG and GG/AA, in the co-dominant model, and GG/AG + AA, in the dominant model, were significantly more frequent in AIS than in control, 7.9% vs. 1.4%,11.3% vs 0% and 16.9% vs 1.4%, respectively. Furthermore, logistic regression analysis identified hypertension (OR (95%CI) = 7.48(2.64–21.22), P